Assay Detail
Binding
CHEMBL1001307
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Inhibition of TNF-induced NF-kappaB-mediated IL6 promoter expression in mouse L929sA cells by luciferase reporter gene assay
5
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | L929sA |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Withaferin a strongly elicits IkappaB kinase beta hyperphosphorylation concomitant with potent inhibition of its kinase activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.14 | 6.60 |
| Efficacy | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL517080 | WITHAFERIN A | 1.0 | 2 | 6.60 |
| CHEMBL338474 | HELENALIN | — | 1 | 5.60 |
| CHEMBL445041 | WITHANOLIDE A | — | 1 | 4.30 |
| CHEMBL382378 | — | — | 1 | 4.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL517080 | WITHAFERIN A | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL338474 | HELENALIN | IC50 | = | 2500.0 | nM | 5.60 |
| CHEMBL445041 | WITHANOLIDE A | IC50 | = | 50000.0 | nM | 4.30 |
| CHEMBL382378 | — | IC50 | = | 90000.0 | nM | 4.05 |
| CHEMBL517080 | WITHAFERIN A | Efficacy | = | 95.0 | % | - |