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Assay Detail

CHEMBL1004110

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at rat mGluR5 expressed in HEK293A cells assessed as glutamate-induced calcium flux preincubated for 140 sec before glutamate challenge by calcium fluorescence assay
40
Total Activities
40
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type HEK293-A
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 5 (CHEMBL2564)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Structure-activity relationships comparing N-(6-methylpyridin-yl)-substituted aryl amides to 2-methyl-6-(substituted-arylethynyl)pyridines or 2-methyl-4-(substituted-arylethynyl)thiazoles as novel metabotropic glutamate receptor subtype 5 antagonists.
Kulkarni SS, Zou MF, Cao J, Deschamps JR, Rodriguez AL, Conn PJ, Newman AH.
J Med Chem (2009) 52 : 3563 -3575
PubMed 19445453 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 7.94 9.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL181483 1 9.38
CHEMBL446543 1 9.09
CHEMBL184995 1 8.92
CHEMBL365403 1 8.52
CHEMBL523693 1 8.51
CHEMBL443979 1 8.46
CHEMBL66654 1 8.45
CHEMBL493569 1 8.34
CHEMBL511603 1 8.32
CHEMBL369310 1 8.28
CHEMBL493773 1 8.21
CHEMBL493774 1 8.14
CHEMBL292065 1 7.87
CHEMBL466006 1 7.86
CHEMBL464372 1 7.66
CHEMBL493568 1 7.60
CHEMBL397121 1 7.01
CHEMBL208927 1 6.31
CHEMBL522507 1 6.19
CHEMBL377829 1 5.73
CHEMBL493777 1 -
CHEMBL377935 1 -
CHEMBL493572 1 -
CHEMBL525384 1 -
CHEMBL493573 1 -
CHEMBL523856 1 -
CHEMBL493570 1 -
CHEMBL493571 1 -
CHEMBL493775 1 -
CHEMBL493776 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL181483 IC50 = 0.415 nM 9.38
CHEMBL446543 IC50 = 0.813 nM 9.09
CHEMBL184995 IC50 = 1.21 nM 8.92
CHEMBL365403 IC50 = 3.05 nM 8.52
CHEMBL523693 IC50 = 3.08 nM 8.51
CHEMBL443979 IC50 = 3.43 nM 8.46
CHEMBL66654 IC50 = 3.54 nM 8.45
CHEMBL493569 IC50 = 4.57 nM 8.34
CHEMBL511603 IC50 = 4.83 nM 8.32
CHEMBL369310 IC50 = 5.24 nM 8.28
CHEMBL493773 IC50 = 6.1 nM 8.21
CHEMBL493774 IC50 = 7.19 nM 8.14
CHEMBL292065 IC50 = 13.6 nM 7.87
CHEMBL466006 IC50 = 13.7 nM 7.86
CHEMBL464372 IC50 = 21.7 nM 7.66
CHEMBL493568 IC50 = 25.3 nM 7.60
CHEMBL397121 IC50 = 98.1 nM 7.01
CHEMBL208927 IC50 = 490.0 nM 6.31
CHEMBL522507 IC50 = 640.0 nM 6.19
CHEMBL377829 IC50 = 1870.0 nM 5.73
CHEMBL468525 IC50 - -
CHEMBL468526 IC50 - -
CHEMBL464550 IC50 - -
CHEMBL501020 IC50 - -
CHEMBL467483 IC50 - -
CHEMBL467484 IC50 - -
CHEMBL467485 IC50 - -
CHEMBL469121 IC50 > 3000.0 nM -
CHEMBL513699 IC50 > 1000.0 nM -
CHEMBL512634 IC50 - -
CHEMBL493776 IC50 - -
CHEMBL493775 IC50 - -
CHEMBL493571 IC50 - -
CHEMBL493570 IC50 > 1000.0 nM -
CHEMBL523856 IC50 - -
CHEMBL493573 IC50 - -
CHEMBL525384 IC50 > 3000.0 nM -
CHEMBL493572 IC50 > 3000.0 nM -
CHEMBL377935 IC50 - -
CHEMBL493777 IC50 - -