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Assay Detail

CHEMBL1005834

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]epibatidine from Aplysia californica AchBP I118M, M116L mutant expressed in HEK293S cells by scintillation proximity assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Aplysia californica
Cell Type HEK293S
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Nicotinic agonist binding site mapped by methionine- and tyrosine-scanning coupled with azidochloropyridinyl photoaffinity labeling.
Tomizawa M, Talley TT, Park JF, Maltby D, Medzihradszky KF, Durkin KA, Cornejo-Bravo JM, Burlingame AL, Casida JE, Taylor P.
J Med Chem (2009) 52 : 3735 -3741
PubMed 19459645 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 3 7.34 8.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL55716 AZIDOEPIBATIDINE 1 8.32
CHEMBL95904 1 7.17
CHEMBL95352 THIACLOPRID OLEFIN 1 6.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL55716 AZIDOEPIBATIDINE Kd = 4.8 nM 8.32
CHEMBL95904 Kd = 68.0 nM 7.17
CHEMBL95352 THIACLOPRID OLEFIN Kd = 299.0 nM 6.52