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Assay Detail

CHEMBL1006407

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse recombinant GARFTase
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Trifunctional purine biosynthetic protein adenosine-3 (CHEMBL3690)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and discovery of high affinity folate receptor-specific glycinamide ribonucleotide formyltransferase inhibitors with antitumor activity.
Deng Y, Wang Y, Cherian C, Hou Z, Buck SA, Matherly LH, Gangjee A.
J Med Chem (2008) 51 : 5052 -5063
PubMed 18680275 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.96 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL365307 1 6.82
CHEMBL34412 LOMETREXOL 2.0 1 6.11
CHEMBL502528 1 5.65
CHEMBL192632 1 5.61
CHEMBL526928 1 5.60
CHEMBL498800 1 -
CHEMBL225072 PEMETREXED 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL365307 IC50 = 150.0 nM 6.82
CHEMBL34412 LOMETREXOL IC50 = 780.0 nM 6.11
CHEMBL502528 IC50 = 2260.0 nM 5.65
CHEMBL192632 IC50 = 2440.0 nM 5.61
CHEMBL526928 IC50 = 2510.0 nM 5.60
CHEMBL498800 IC50 > 20000.0 nM -
CHEMBL225072 PEMETREXED IC50 > 20000.0 nM -