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Assay Detail

CHEMBL1007110

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to Trypanosoma cruzi CYP51 expressed in Escherichia coli by spectral titration
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma cruzi
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Sterol 14-alpha demethylase (CHEMBL1075110)
Type SINGLE PROTEIN
Organism Trypanosoma cruzi (strain CL Brener)

Publication

Indomethacin amides as a novel molecular scaffold for targeting Trypanosoma cruzi sterol 14alpha-demethylase.
Konkle ME, Hargrove TY, Kleshchenko YY, von Kries JP, Ridenour W, Uddin MJ, Caprioli RM, Marnett LJ, Nes WD, Villalta F, Waterman MR, Lepesheva GI.
J Med Chem (2009) 52 : 2846 -2853
PubMed 19354253 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 13 6.21 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL473679 1 6.82
CHEMBL474704 1 6.62
CHEMBL470642 1 6.58
CHEMBL329480 1 6.54
CHEMBL469820 1 6.44
CHEMBL484729 1 6.43
CHEMBL507264 1 6.36
CHEMBL521330 1 6.27
CHEMBL470641 1 6.25
CHEMBL474703 1 6.12
CHEMBL469819 1 5.64
CHEMBL484730 1 5.48
CHEMBL519512 1 5.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL473679 Kd = 150.0 nM 6.82
CHEMBL474704 Kd = 240.0 nM 6.62
CHEMBL470642 Kd = 260.0 nM 6.58
CHEMBL329480 Kd = 290.0 nM 6.54
CHEMBL469820 Kd = 360.0 nM 6.44
CHEMBL484729 Kd = 370.0 nM 6.43
CHEMBL507264 Kd = 440.0 nM 6.36
CHEMBL521330 Kd = 540.0 nM 6.27
CHEMBL470641 Kd = 560.0 nM 6.25
CHEMBL474703 Kd = 750.0 nM 6.12
CHEMBL469819 Kd = 2310.0 nM 5.64
CHEMBL484730 Kd = 3300.0 nM 5.48
CHEMBL519512 Kd = 7600.0 nM 5.12