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Assay Detail

CHEMBL1008005

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Functional
Agonist activity at human recombinant motilin receptor expressed in CHO cells assessed as increase in intracellular calcium by FLIPR assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Motilin receptor (CHEMBL2203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of N-(3-fluorophenyl)-1-[(4-([(3S)-3-methyl-1-piperazinyl]methyl)phenyl)acetyl]-4-piperidinamine (GSK962040), the first small molecule motilin receptor agonist clinical candidate.
Westaway SM, Brown SL, Fell SC, Johnson CN, MacPherson DT, Mitchell DJ, Myatt JW, Stanway SJ, Seal JT, Stemp G, Thompson M, Lawless K, McKay F, Muir AI, Barford JM, Cluff C, Mahmood SR, Matthews KL, Mohamed S, Smith B, Stevens AJ, Bolton VJ, Jarvie EM, Sanger GJ.
J Med Chem (2009) 52 : 1180 -1189
PubMed 19191554 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 7.89 10.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL525634 1 10.40
CHEMBL473921 1 8.40
CHEMBL489480 1 8.40
CHEMBL489094 1 8.00
CHEMBL489095 CAMICINAL 3.0 1 7.90
CHEMBL523933 1 7.70
CHEMBL474130 1 7.60
CHEMBL489297 1 7.50
CHEMBL489479 1 7.50
CHEMBL474934 1 7.40
CHEMBL514475 1 7.30
CHEMBL532 ERYTHROMYCIN 4.0 1 7.30
CHEMBL474129 1 7.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL525634 EC50 = 0.03981 nM 10.40
CHEMBL473921 EC50 = 3.981 nM 8.40
CHEMBL489480 EC50 = 3.981 nM 8.40
CHEMBL489094 EC50 = 10.0 nM 8.00
CHEMBL489095 CAMICINAL EC50 = 12.59 nM 7.90
CHEMBL523933 EC50 = 19.95 nM 7.70
CHEMBL474130 EC50 = 25.12 nM 7.60
CHEMBL489297 EC50 = 31.62 nM 7.50
CHEMBL489479 EC50 = 31.62 nM 7.50
CHEMBL474934 EC50 = 39.81 nM 7.40
CHEMBL514475 EC50 = 50.12 nM 7.30
CHEMBL532 ERYTHROMYCIN EC50 = 50.12 nM 7.30
CHEMBL474129 EC50 = 63.1 nM 7.20