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Assay Detail

CHEMBL1008931

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human GnRH receptor expressed in RBL1 cells assessed as inhibition of GnRH-stimulated inositol phosphate production
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type RBL-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of sodium R-(+)-4-{2-[5-(2-fluoro-3-methoxyphenyl)-3-(2-fluoro-6-[trifluoromethyl]benzyl)-4-methyl-2,6-dioxo-3,6-dihydro-2H-pyrimidin-1-yl]-1-phenylethylamino}butyrate (elagolix), a potent and orally available nonpeptide antagonist of the human gonadotropin-releasing hormone receptor.
Chen C, Wu D, Guo Z, Xie Q, Reinhart GJ, Madan A, Wen J, Chen T, Huang CQ, Chen M, Chen Y, Tucci FC, Rowbottom M, Pontillo J, Zhu YF, Wade W, Saunders J, Bozigian H, Struthers RS.
J Med Chem (2008) 51 : 7478 -7485
PubMed 19006286 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 8.34 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL468551 1 9.30
CHEMBL502182 ELAGOLIX SODIUM 4.0 1 8.82
CHEMBL179691 1 8.55
CHEMBL502341 1 8.49
CHEMBL506877 1 7.57
CHEMBL509075 1 7.33
CHEMBL503705 1 -
CHEMBL510538 1 -
CHEMBL501329 1 -
CHEMBL509440 1 -
CHEMBL510285 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL468551 IC50 = 0.5 nM 9.30
CHEMBL502182 ELAGOLIX SODIUM IC50 = 1.5 nM 8.82
CHEMBL179691 IC50 = 2.8 nM 8.55
CHEMBL502341 IC50 = 3.2 nM 8.49
CHEMBL506877 IC50 = 27.0 nM 7.57
CHEMBL509075 IC50 = 47.0 nM 7.33
CHEMBL503705 IC50 - -
CHEMBL510538 IC50 - -
CHEMBL501329 IC50 - -
CHEMBL509440 IC50 - -
CHEMBL510285 IC50 - -