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Assay Detail

CHEMBL1010576

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant Arg235 region of BACE1 by FRET assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 1 (CHEMBL4822)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Significance of interactions of BACE1-Arg235 with its ligands and design of BACE1 inhibitors with P2 pyridine scaffold.
Hamada Y, Ohta H, Miyamoto N, Sarma D, Hamada T, Nakanishi T, Yamasaki M, Yamani A, Ishiura S, Kiso Y.
Bioorg Med Chem Lett (2009) 19 : 2435 -2439
PubMed 19345096 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.47 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL453211 1 8.05
CHEMBL507541 1 8.00
CHEMBL501242 1 7.89
CHEMBL445281 1 7.82
CHEMBL448015 1 7.66
CHEMBL504917 1 7.62
CHEMBL400043 1 7.30
CHEMBL399840 1 7.02
CHEMBL399839 1 6.85
CHEMBL253865 1 6.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL453211 IC50 = 9.0 nM 8.05
CHEMBL507541 IC50 = 10.0 nM 8.00
CHEMBL501242 IC50 = 13.0 nM 7.89
CHEMBL445281 IC50 = 15.0 nM 7.82
CHEMBL448015 IC50 = 22.0 nM 7.66
CHEMBL504917 IC50 = 24.0 nM 7.62
CHEMBL400043 IC50 = 50.0 nM 7.30
CHEMBL399840 IC50 = 96.0 nM 7.02
CHEMBL399839 IC50 = 140.0 nM 6.85
CHEMBL253865 IC50 = 360.0 nM 6.44