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Assay Detail

CHEMBL1011051

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Binding
Inhibition of PKBgamma using 100 uM ATP and 30 uM TAMRA-kemptide substrate by fluorometric TLC assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Intermediate

Target

RAC-gamma serine/threonine-protein kinase (CHEMBL4816)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural analysis of ARC-type inhibitor (ARC-1034) binding to protein kinase A catalytic subunit and rational design of bisubstrate analogue inhibitors of basophilic protein kinases.
Lavogina D, Lust M, Viil I, König N, Raidaru G, Rogozina J, Enkvist E, Uri A, Bossemeyer D.
J Med Chem (2009) 52 : 308 -321
PubMed 19143565 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.75 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL498924 1 8.62
CHEMBL525385 1 7.84
CHEMBL524516 1 7.25
CHEMBL1315166 1 6.18
CHEMBL297394 1 6.11
CHEMBL501706 1 5.79
CHEMBL505949 1 5.43
CHEMBL495766 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL498924 IC50 = 2.38 nM 8.62
CHEMBL525385 IC50 = 14.6 nM 7.84
CHEMBL524516 IC50 = 56.6 nM 7.25
CHEMBL1315166 IC50 = 662.0 nM 6.18
CHEMBL297394 IC50 = 774.0 nM 6.11
CHEMBL501706 IC50 = 1610.0 nM 5.79
CHEMBL505949 IC50 = 3750.0 nM 5.43
CHEMBL495766 IC50 > 1000000.0 nM -