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Assay Detail

CHEMBL1011627

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PDE4
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Phosphodiesterase 4 (CHEMBL2093863)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Discovery of (S)-N-[2-[1-(3-ethoxy-4-methoxyphenyl)-2-methanesulfonylethyl]-1,3-dioxo-2,3-dihydro-1H-isoindol-4-yl] acetamide (apremilast), a potent and orally active phosphodiesterase 4 and tumor necrosis factor-alpha inhibitor.
Man HW, Schafer P, Wong LM, Patterson RT, Corral LG, Raymon H, Blease K, Leisten J, Shirley MA, Tang Y, Babusis DM, Chen R, Stirling D, Muller GW.
J Med Chem (2009) 52 : 1522 -1524
PubMed 19256507 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.66 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL193240 ROFLUMILAST 4.0 1 9.52
CHEMBL313439 1 7.40
CHEMBL473205 1 7.17
CHEMBL514800 APREMILAST 4.0 1 7.13
CHEMBL475165 1 7.09
CHEMBL474556 1 6.92
CHEMBL474557 1 6.92
CHEMBL472963 1 6.89
CHEMBL514339 1 6.66
CHEMBL88475 1 6.66
CHEMBL475164 1 6.58
CHEMBL315529 1 6.47
CHEMBL515728 1 6.47
CHEMBL473204 1 6.21
CHEMBL508838 1 6.16
CHEMBL516063 1 6.15
CHEMBL515398 1 6.09
CHEMBL88153 CDC-801 2.0 1 5.96
CHEMBL473983 1 5.89
CHEMBL90345 1 5.80
CHEMBL419135 1 5.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL193240 ROFLUMILAST IC50 = 0.3 nM 9.52
CHEMBL313439 IC50 = 40.0 nM 7.40
CHEMBL473205 IC50 = 68.0 nM 7.17
CHEMBL514800 APREMILAST IC50 = 74.0 nM 7.13
CHEMBL475165 IC50 = 82.0 nM 7.09
CHEMBL474556 IC50 = 120.0 nM 6.92
CHEMBL474557 IC50 = 120.0 nM 6.92
CHEMBL472963 IC50 = 130.0 nM 6.89
CHEMBL514339 IC50 = 220.0 nM 6.66
CHEMBL88475 IC50 = 220.0 nM 6.66
CHEMBL475164 IC50 = 260.0 nM 6.58
CHEMBL315529 IC50 = 340.0 nM 6.47
CHEMBL515728 IC50 = 340.0 nM 6.47
CHEMBL473204 IC50 = 610.0 nM 6.21
CHEMBL508838 IC50 = 690.0 nM 6.16
CHEMBL516063 IC50 = 710.0 nM 6.15
CHEMBL515398 IC50 = 820.0 nM 6.09
CHEMBL88153 CDC-801 IC50 = 1100.0 nM 5.96
CHEMBL473983 IC50 = 1300.0 nM 5.89
CHEMBL90345 IC50 = 1600.0 nM 5.80
CHEMBL419135 IC50 = 1900.0 nM 5.72