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Assay Detail

CHEMBL1012214

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]methylspiperone form human cloned 5HT2A receptor expressed in african green monkey COS7 cells by scintillation spectroscopy
10
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

New antiinfective and human 5-HT2 receptor binding natural and semisynthetic compounds from the Jamaican sponge Smenospongia aurea.
Hu JF, Schetz JA, Kelly M, Peng JN, Ang KK, Flotow H, Leong CY, Ng SB, Buss AD, Wilkins SP, Hamann MT.
J Nat Prod (2002) 65 : 476 -480
PubMed 11975483 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 5.99 6.50
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL39 SEROTONIN 3.0 1 6.50
CHEMBL463250 1 5.77
CHEMBL463249 1 5.70
CHEMBL511879 2 -
CHEMBL504264 AUREOL 1 -
CHEMBL463442 1 -
CHEMBL517151 1 -
CHEMBL457256 1 -
CHEMBL457078 AUREOL ACETATE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL39 SEROTONIN Ki = 320.0 nM 6.50
CHEMBL463250 Ki = 1700.0 nM 5.77
CHEMBL463249 Ki = 2000.0 nM 5.70
CHEMBL511879 Ki > 100000.0 nM -
CHEMBL504264 AUREOL Ki - -
CHEMBL463442 Ki - -
CHEMBL517151 Ki - -
CHEMBL457256 Ki - -
CHEMBL457078 AUREOL ACETATE Ki - -
CHEMBL511879 Inhibition = 10.0 % -