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Assay Detail

CHEMBL1012734

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]AB-MECA from human cloned adenosine A3 receptor expressed in CHO cells
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A3 (CHEMBL256)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyrido[2,3-e]-1,2,4-triazolo[4,3-a]pyrazin-1-one as a new scaffold to develop potent and selective human A3 adenosine receptor antagonists. Synthesis, pharmacological evaluation, and ligand-receptor modeling studies.
Colotta V, Lenzi O, Catarzi D, Varano F, Filacchioni G, Martini C, Trincavelli L, Ciampi O, Pugliese AM, Traini C, Pedata F, Morizzo E, Moro S.
J Med Chem (2009) 52 : 2407 -2419
PubMed 19301821 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 27 7.01 8.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL475791 1 8.48
CHEMBL474016 1 8.34
CHEMBL333799 1 8.33
CHEMBL421420 1 8.32
CHEMBL475026 1 8.11
CHEMBL473649 1 8.08
CHEMBL473839 1 7.93
CHEMBL473648 1 7.81
CHEMBL378276 1 7.66
CHEMBL516049 1 7.39
CHEMBL120714 1 7.33
CHEMBL473650 1 7.15
CHEMBL492955 1 6.94
CHEMBL514156 1 6.86
CHEMBL474977 1 6.80
CHEMBL212639 1 6.66
CHEMBL514196 1 6.60
CHEMBL508031 1 6.55
CHEMBL492356 1 6.55
CHEMBL516372 1 6.47
CHEMBL377781 1 6.47
CHEMBL515433 1 6.31
CHEMBL475792 1 6.23
CHEMBL474636 1 6.18
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] 1 5.89
CHEMBL514155 1 5.88
CHEMBL1355736 THEOPHYLLINE 4.0 1 4.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL475791 Ki = 3.3 nM 8.48
CHEMBL474016 Ki = 4.54 nM 8.34
CHEMBL333799 Ki = 4.7 nM 8.33
CHEMBL421420 Ki = 4.75 nM 8.32
CHEMBL475026 Ki = 7.75 nM 8.11
CHEMBL473649 Ki = 8.4 nM 8.08
CHEMBL473839 Ki = 11.7 nM 7.93
CHEMBL473648 Ki = 15.5 nM 7.81
CHEMBL378276 Ki = 22.0 nM 7.66
CHEMBL516049 Ki = 41.0 nM 7.39
CHEMBL120714 Ki = 47.0 nM 7.33
CHEMBL473650 Ki = 70.3 nM 7.15
CHEMBL492955 Ki = 116.0 nM 6.94
CHEMBL514156 Ki = 138.0 nM 6.86
CHEMBL474977 Ki = 158.0 nM 6.80
CHEMBL212639 Ki = 217.0 nM 6.66
CHEMBL514196 Ki = 251.0 nM 6.60
CHEMBL508031 Ki = 279.0 nM 6.55
CHEMBL492356 Ki = 281.0 nM 6.55
CHEMBL516372 Ki = 335.0 nM 6.47
CHEMBL377781 Ki = 342.0 nM 6.47
CHEMBL515433 Ki = 490.0 nM 6.31
CHEMBL475792 Ki = 590.0 nM 6.23
CHEMBL474636 Ki = 656.0 nM 6.18
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] Ki = 1300.0 nM 5.89
CHEMBL514155 Ki = 1335.0 nM 5.88
CHEMBL1355736 THEOPHYLLINE Ki = 86000.0 nM 4.07