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Assay Detail

CHEMBL1014542

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Functional
Agonist activity at NTR1 in human HT-29 cells assessed as increase in intracellular calcium concentration by FLIPR assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neurotensin receptor type 1 (CHEMBL4123)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Comparison of N-terminal modifications on neurotensin(8-13) analogues correlates peptide stability but not binding affinity with in vivo efficacy.
Orwig KS, Lassetter MR, Hadden MK, Dix TA.
J Med Chem (2009) 52 : 1803 -1813
PubMed 19290594 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 19 7.74 9.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL504768 1 9.11
CHEMBL468951 1 9.07
CHEMBL342252 1 9.00
CHEMBL505695 1 8.73
CHEMBL344337 1 8.72
CHEMBL407196 NEUROTENSIN 1 8.70
CHEMBL505906 1 8.21
CHEMBL499673 1 8.19
CHEMBL453924 1 8.14
CHEMBL501575 1 8.11
CHEMBL447400 1 8.10
CHEMBL450258 1 7.59
CHEMBL508507 1 7.24
CHEMBL508380 1 6.82
CHEMBL266571 1 6.70
CHEMBL445035 1 6.66
CHEMBL500734 1 6.12
CHEMBL500173 1 6.11
CHEMBL501956 1 5.73

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL504768 EC50 = 0.78 nM 9.11
CHEMBL468951 EC50 = 0.853 nM 9.07
CHEMBL342252 EC50 = 1.0 nM 9.00
CHEMBL505695 EC50 = 1.85 nM 8.73
CHEMBL344337 EC50 = 1.89 nM 8.72
CHEMBL407196 NEUROTENSIN EC50 = 1.98 nM 8.70
CHEMBL505906 EC50 = 6.21 nM 8.21
CHEMBL499673 EC50 = 6.44 nM 8.19
CHEMBL453924 EC50 = 7.28 nM 8.14
CHEMBL501575 EC50 = 7.76 nM 8.11
CHEMBL447400 EC50 = 7.96 nM 8.10
CHEMBL450258 EC50 = 25.8 nM 7.59
CHEMBL508507 EC50 = 57.7 nM 7.24
CHEMBL508380 EC50 = 151.0 nM 6.82
CHEMBL266571 EC50 = 200.0 nM 6.70
CHEMBL445035 EC50 = 217.0 nM 6.66
CHEMBL500734 EC50 = 764.0 nM 6.12
CHEMBL500173 EC50 = 783.0 nM 6.11
CHEMBL501956 EC50 = 1860.0 nM 5.73