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Assay Detail

CHEMBL1016589

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of glutamate-induced depolarization in human EAAT3 expressed in HEK293 cells by FMP assay
24
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Excitatory amino acid transporter 3 (CHEMBL2721)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chemo-enzymatic synthesis of (2S,4R)-2-amino-4-(3-(2,2-diphenylethylamino)-3-oxopropyl)pentanedioic acid: a novel selective inhibitor of human excitatory amino acid transporter subtype 2.
Sagot E, Jensen AA, Pickering DS, Pu X, Umberti M, Stensbøl TB, Nielsen B, Assaf Z, Aboab B, Bolte J, Gefflaut T, Bunch L.
J Med Chem (2008) 51 : 4085 -4092
PubMed 18578477 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.02 5.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL475341 2 5.03
CHEMBL500448 2 -
CHEMBL279561 DIHYDROKAINATE 2 -
CHEMBL480169 2 -
CHEMBL518313 2 -
CHEMBL494878 2 -
CHEMBL500042 2 -
CHEMBL479804 2 -
CHEMBL482080 2 -
CHEMBL482081 2 -
CHEMBL479797 2 -
CHEMBL446256 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL475341 IC50 = 9300.0 nM 5.03
CHEMBL475341 IC50 = 10000.0 nM 5.00
CHEMBL500448 IC50 = 3162277.66 nM -
CHEMBL279561 DIHYDROKAINATE IC50 > 3000000.0 nM -
CHEMBL279561 DIHYDROKAINATE IC50 > 3162277.66 nM -
CHEMBL480169 IC50 = 1000000.0 nM -
CHEMBL480169 IC50 = 1000000.0 nM -
CHEMBL518313 IC50 = 1000000.0 nM -
CHEMBL518313 IC50 = 1000000.0 nM -
CHEMBL494878 IC50 = 3000000.0 nM -
CHEMBL494878 IC50 = 3162277.66 nM -
CHEMBL500448 IC50 = 3000000.0 nM -
CHEMBL500042 IC50 > 1000000.0 nM -
CHEMBL479804 IC50 > 3000000.0 nM -
CHEMBL479804 IC50 = 3162277.66 nM -
CHEMBL482080 IC50 > 1000000.0 nM -
CHEMBL482080 IC50 > 1000000.0 nM -
CHEMBL482081 IC50 = 3000000.0 nM -
CHEMBL482081 IC50 = 3162277.66 nM -
CHEMBL479797 IC50 > 3000000.0 nM -
CHEMBL479797 IC50 > 3162277.66 nM -
CHEMBL446256 IC50 = 3000000.0 nM -
CHEMBL446256 IC50 = 3162277.66 nM -
CHEMBL500042 IC50 > 1000000.0 nM -