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Assay Detail

CHEMBL1017447

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of LFA1 expressed in human JY cells interaction with ICAM1-IG expressed in human HeLa cell monolayer after 45 mins by cell adhesion assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type JY
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Integrin alpha-L (CHEMBL1803)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cucurbitacins, cell adhesion inhibitors from Conobea scoparioides.
Musza LL, Speight P, McElhiney S, Barrow CJ, Gillum AM, Cooper R, Killar LM.
J Nat Prod (1994) 57 : 1498 -1502
PubMed 7852999 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.29 6.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL455056 CUCURBITACIN E 1 6.75
CHEMBL507237 CUCURBITACIN B 1 6.52
CHEMBL387737 CUCURBITACIN I 1 6.02
CHEMBL493646 CUCURBITACIN D 1 5.87
CHEMBL564629 CUCURBITACIN R 1 -
CHEMBL480111 CUCURBITACIN L 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL455056 CUCURBITACIN E IC50 = 180.0 nM 6.75
CHEMBL507237 CUCURBITACIN B IC50 = 300.0 nM 6.52
CHEMBL387737 CUCURBITACIN I IC50 = 950.0 nM 6.02
CHEMBL493646 CUCURBITACIN D IC50 = 1360.0 nM 5.87
CHEMBL564629 CUCURBITACIN R IC50 > 50000.0 nM -
CHEMBL480111 CUCURBITACIN L IC50 > 50000.0 nM -