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Assay Detail

CHEMBL1017975

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at rat P2X3 receptor expressed in CHO cells by FLIPR
28
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 3 (CHEMBL4824)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Identification and SAR of novel diaminopyrimidines. Part 2: The discovery of RO-51, a potent and selective, dual P2X(3)/P2X(2/3) antagonist for the treatment of pain.
Jahangir A, Alam M, Carter DS, Dillon MP, Bois DJ, Ford AP, Gever JR, Lin C, Wagner PJ, Zhai Y, Zira J.
Bioorg Med Chem Lett (2009) 19 : 1632 -1635
PubMed 19231178 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 7.39 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL494161 1 8.70
CHEMBL523173 1 8.20
CHEMBL494353 1 8.20
CHEMBL494160 1 8.10
CHEMBL494582 1 8.00
CHEMBL492933 1 8.00
CHEMBL526307 2 8.00
CHEMBL494158 1 8.00
CHEMBL492300 1 8.00
CHEMBL494159 1 7.90
CHEMBL492907 1 7.80
CHEMBL492934 1 7.80
CHEMBL443930 1 7.30
CHEMBL492745 1 7.20
CHEMBL492703 1 7.20
CHEMBL493741 1 7.10
CHEMBL492299 1 7.00
CHEMBL494581 1 7.00
CHEMBL493547 1 6.90
CHEMBL493546 1 6.80
CHEMBL492935 1 6.80
CHEMBL523000 1 6.60
CHEMBL492729 1 6.60
CHEMBL492789 1 6.40
CHEMBL492744 1 6.40
CHEMBL493740 1 5.60
CHEMBL523840 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL494161 IC50 = 1.995 nM 8.70
CHEMBL523173 IC50 = 6.31 nM 8.20
CHEMBL494353 IC50 = 6.31 nM 8.20
CHEMBL494160 IC50 = 7.943 nM 8.10
CHEMBL494582 IC50 = 10.0 nM 8.00
CHEMBL492300 IC50 = 10.0 nM 8.00
CHEMBL492933 IC50 = 10.0 nM 8.00
CHEMBL526307 IC50 = 10.0 nM 8.00
CHEMBL494158 IC50 = 10.0 nM 8.00
CHEMBL494159 IC50 = 12.59 nM 7.90
CHEMBL526307 IC50 = 13.0 nM 7.89
CHEMBL492907 IC50 = 15.85 nM 7.80
CHEMBL492934 IC50 = 15.85 nM 7.80
CHEMBL443930 IC50 = 50.12 nM 7.30
CHEMBL492745 IC50 = 63.1 nM 7.20
CHEMBL492703 IC50 = 63.1 nM 7.20
CHEMBL493741 IC50 = 79.43 nM 7.10
CHEMBL492299 IC50 = 100.0 nM 7.00
CHEMBL494581 IC50 = 100.0 nM 7.00
CHEMBL493547 IC50 = 125.89 nM 6.90
CHEMBL493546 IC50 = 158.49 nM 6.80
CHEMBL492935 IC50 = 158.49 nM 6.80
CHEMBL523000 IC50 = 251.19 nM 6.60
CHEMBL492729 IC50 = 251.19 nM 6.60
CHEMBL492789 IC50 = 398.11 nM 6.40
CHEMBL492744 IC50 = 398.11 nM 6.40
CHEMBL493740 IC50 = 2511.89 nM 5.60
CHEMBL523840 IC50 > 10000.0 nM -