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Assay Detail

CHEMBL1020340

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIV1 reverse transcriptase p66/p51 K65R mutant expressed in Escherichia coli using calf thymus DNA substrate
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 reverse transcriptase (CHEMBL247)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

In vitro suppression of K65R reverse transcriptase-mediated tenofovir- and adefovir-5'-diphosphate resistance conferred by the boranophosphonate derivatives.
Frangeul A, Barral K, Alvarez K, Canard B.
Antimicrob Agents Chemother (2007) 51 : 3162 -3167
PubMed 17620380 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.65 4.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2448397 1 4.92
CHEMBL2448398 1 4.75
CHEMBL1162433 ADEFOVIR DIPHOSPHATE -1.0 1 4.28
CHEMBL1236349 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2448397 IC50 = 12000.0 nM 4.92
CHEMBL2448398 IC50 = 18000.0 nM 4.75
CHEMBL1162433 ADEFOVIR DIPHOSPHATE IC50 = 52000.0 nM 4.28
CHEMBL1236349 IC50 = 170000.0 nM -