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Assay Detail

CHEMBL1027267

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse MC1R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocyte-stimulating hormone receptor (CHEMBL4077)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Melanocortin tetrapeptide Ac-His-DPhe-Arg-Trp-NH2 modified at the para position of the benzyl side chain (DPhe): importance for mouse melanocortin-3 receptor agonist versus antagonist activity.
Proneth B, Pogozheva ID, Portillo FP, Mosberg HI, Haskell-Luevano C.
J Med Chem (2008) 51 : 5585 -5593
PubMed 18800761 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 6.91 10.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 10.06
CHEMBL491870 1 7.42
CHEMBL322610 1 7.39
CHEMBL502093 1 7.39
CHEMBL444219 1 7.27
CHEMBL454736 1 7.03
CHEMBL488355 1 7.00
CHEMBL264190 1 6.95
CHEMBL501394 1 6.85
CHEMBL503449 1 6.77
CHEMBL455826 1 6.75
CHEMBL448536 1 6.55
CHEMBL510687 1 6.50
CHEMBL501679 1 6.27
CHEMBL451423 1 5.99
CHEMBL500516 1 5.64
CHEMBL105113 1 5.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE EC50 = 0.088 nM 10.06
CHEMBL491870 EC50 = 38.2 nM 7.42
CHEMBL322610 EC50 = 40.7 nM 7.39
CHEMBL502093 EC50 = 40.8 nM 7.39
CHEMBL444219 EC50 = 53.7 nM 7.27
CHEMBL454736 EC50 = 92.8 nM 7.03
CHEMBL488355 EC50 = 100.0 nM 7.00
CHEMBL264190 EC50 = 112.0 nM 6.95
CHEMBL501394 EC50 = 140.0 nM 6.85
CHEMBL503449 EC50 = 170.0 nM 6.77
CHEMBL455826 EC50 = 180.0 nM 6.75
CHEMBL448536 EC50 = 280.0 nM 6.55
CHEMBL510687 EC50 = 320.0 nM 6.50
CHEMBL501679 EC50 = 540.0 nM 6.27
CHEMBL451423 EC50 = 1030.0 nM 5.99
CHEMBL500516 EC50 = 2300.0 nM 5.64
CHEMBL105113 EC50 = 2600.0 nM 5.58