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Assay Detail

CHEMBL1027275

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse MC4R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 4 (CHEMBL3719)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Melanocortin tetrapeptide Ac-His-DPhe-Arg-Trp-NH2 modified at the para position of the benzyl side chain (DPhe): importance for mouse melanocortin-3 receptor agonist versus antagonist activity.
Proneth B, Pogozheva ID, Portillo FP, Mosberg HI, Haskell-Luevano C.
J Med Chem (2008) 51 : 5585 -5593
PubMed 18800761 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 7.97 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL491870 1 9.40
CHEMBL444219 1 9.35
CHEMBL502093 1 8.97
CHEMBL264190 1 8.64
CHEMBL448536 1 8.55
CHEMBL501394 1 8.47
CHEMBL322610 1 8.40
CHEMBL503449 1 8.25
CHEMBL510687 1 8.06
CHEMBL501679 1 7.89
CHEMBL451423 1 7.89
CHEMBL455826 1 7.20
CHEMBL500516 1 6.66
CHEMBL105113 1 6.41
CHEMBL488355 1 5.36
CHEMBL441738 AFAMELANOTIDE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL491870 EC50 = 0.4 nM 9.40
CHEMBL444219 EC50 = 0.45 nM 9.35
CHEMBL502093 EC50 = 1.07 nM 8.97
CHEMBL264190 EC50 = 2.28 nM 8.64
CHEMBL448536 EC50 = 2.84 nM 8.55
CHEMBL501394 EC50 = 3.4 nM 8.47
CHEMBL322610 EC50 = 3.94 nM 8.40
CHEMBL503449 EC50 = 5.65 nM 8.25
CHEMBL510687 EC50 = 8.64 nM 8.06
CHEMBL501679 EC50 = 12.8 nM 7.89
CHEMBL451423 EC50 = 13.0 nM 7.89
CHEMBL455826 EC50 = 63.8 nM 7.20
CHEMBL500516 EC50 = 220.0 nM 6.66
CHEMBL105113 EC50 = 390.0 nM 6.41
CHEMBL488355 EC50 = 4360.0 nM 5.36
CHEMBL441738 AFAMELANOTIDE EC50 = 0.12 nM -