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Assay Detail

CHEMBL1028495

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]cytisine from alpha4beta2 nicotinic acetylcholine receptor in rat brain cortex membrane homogenates
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Synthesis, binding, and modeling studies of new cytisine derivatives, as ligands for neuronal nicotinic acetylcholine receptor subtypes.
Tasso B, Canu Boido C, Terranova E, Gotti C, Riganti L, Clementi F, Artali R, Bombieri G, Meneghetti F, Sparatore F.
J Med Chem (2009) 52 : 4345 -4357
PubMed 19548687 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 7.18 9.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL497939 CYTISINICLINE 3.0 1 9.47
CHEMBL190851 1 8.49
CHEMBL502030 1 8.14
CHEMBL502295 1 7.89
CHEMBL500426 1 7.38
CHEMBL519673 1 7.36
CHEMBL497946 1 7.11
CHEMBL526554 1 6.76
CHEMBL524813 1 6.74
CHEMBL526340 1 6.21
CHEMBL483803 1 6.17
CHEMBL508190 1 6.00
CHEMBL499636 1 5.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL497939 CYTISINICLINE Ki = 0.34 nM 9.47
CHEMBL190851 Ki = 3.2 nM 8.49
CHEMBL502030 Ki = 7.2 nM 8.14
CHEMBL502295 Ki = 13.0 nM 7.89
CHEMBL500426 Ki = 42.0 nM 7.38
CHEMBL519673 Ki = 43.5 nM 7.36
CHEMBL497946 Ki = 78.0 nM 7.11
CHEMBL526554 Ki = 172.0 nM 6.76
CHEMBL524813 Ki = 183.0 nM 6.74
CHEMBL526340 Ki = 611.0 nM 6.21
CHEMBL483803 Ki = 670.0 nM 6.17
CHEMBL508190 Ki = 1000.0 nM 6.00
CHEMBL499636 Ki = 2222.0 nM 5.65