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Assay Detail

CHEMBL1031973

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of histidine-tagged Bacillus anthracis Sterne recombinant DHFR expressed in Escherichia coli BL21 Star (DE3)pLysS up to 3 mins
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bacillus anthracis
Confidence 1 — Organism
Curated By Autocuration

Target

Bacillus anthracis (CHEMBL613904)
Type ORGANISM
Organism Bacillus anthracis

Publication

In vitro efficacy of new antifolates against trimethoprim-resistant Bacillus anthracis.
Barrow EW, Dreier J, Reinelt S, Bourne PC, Barrow WW.
Antimicrob Agents Chemother (2007) 51 : 4447 -4452
PubMed 17875993 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.84 7.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL565020 1 7.34
CHEMBL560502 1 7.27
CHEMBL559830 1 7.16
CHEMBL564880 1 7.04
CHEMBL560702 1 7.02
CHEMBL537958 1 7.01
CHEMBL549882 1 6.92
CHEMBL550083 1 6.82
CHEMBL540217 1 6.77
CHEMBL562228 1 6.77
CHEMBL561644 1 6.70
CHEMBL551038 1 6.58
CHEMBL565059 1 6.52
CHEMBL537959 1 6.40
CHEMBL539445 1 6.22
CHEMBL565218 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL565020 IC50 = 46.0 nM 7.34
CHEMBL560502 IC50 = 54.0 nM 7.27
CHEMBL559830 IC50 = 69.0 nM 7.16
CHEMBL564880 IC50 = 92.0 nM 7.04
CHEMBL560702 IC50 = 96.0 nM 7.02
CHEMBL537958 IC50 = 97.0 nM 7.01
CHEMBL549882 IC50 = 120.0 nM 6.92
CHEMBL550083 IC50 = 150.0 nM 6.82
CHEMBL540217 IC50 = 170.0 nM 6.77
CHEMBL562228 IC50 = 170.0 nM 6.77
CHEMBL561644 IC50 = 200.0 nM 6.70
CHEMBL551038 IC50 = 260.0 nM 6.58
CHEMBL565059 IC50 = 300.0 nM 6.52
CHEMBL537959 IC50 = 400.0 nM 6.40
CHEMBL539445 IC50 = 600.0 nM 6.22
CHEMBL565218 IC50 - -