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Assay Detail

CHEMBL1031977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Bacillus cereus recombinant DHFR expressed in Escherichia coli BL21(DE3) assessed as enzyme-dependent NADPH consumption
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bacillus cereus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

In vitro efficacy of new antifolates against trimethoprim-resistant Bacillus anthracis.
Barrow EW, Dreier J, Reinelt S, Bourne PC, Barrow WW.
Antimicrob Agents Chemother (2007) 51 : 4447 -4452
PubMed 17875993 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.18 6.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL560702 1 6.57
CHEMBL550083 1 6.57
CHEMBL551038 1 6.57
CHEMBL561644 1 6.57
CHEMBL565020 1 6.57
CHEMBL565059 1 6.57
CHEMBL549882 1 6.57
CHEMBL562228 1 6.57
CHEMBL560502 1 6.57
CHEMBL565218 1 4.43
CHEMBL564880 1 4.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL560702 IC50 = 270.0 nM 6.57
CHEMBL550083 IC50 = 270.0 nM 6.57
CHEMBL551038 IC50 = 270.0 nM 6.57
CHEMBL561644 IC50 = 270.0 nM 6.57
CHEMBL565020 IC50 = 270.0 nM 6.57
CHEMBL565059 IC50 = 270.0 nM 6.57
CHEMBL549882 IC50 = 270.0 nM 6.57
CHEMBL562228 IC50 = 270.0 nM 6.57
CHEMBL560502 IC50 = 270.0 nM 6.57
CHEMBL565218 IC50 = 37000.0 nM 4.43
CHEMBL564880 IC50 = 37000.0 nM 4.43