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Assay Detail

CHEMBL1032747

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]dopamine uptake at human dopamine transporter expressed in mouse N2A cells by scintillation counting
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type N2a
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Sodium-dependent dopamine transporter (CHEMBL238)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A novel photoaffinity ligand for the dopamine transporter based on pyrovalerone.
Lapinsky DJ, Aggarwal S, Huang Y, Surratt CK, Lever JR, Foster JD, Vaughan RA.
Bioorg Med Chem (2009) 17 : 3770 -3774
PubMed 19442525 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.96 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL555491 1 8.15
CHEMBL201960 PYROVALERONE 4.0 1 7.50
CHEMBL553937 1 6.76
CHEMBL558086 1 6.58
CHEMBL894 BUPROPION 4.0 1 5.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL555491 IC50 = 7.0 nM 8.15
CHEMBL201960 PYROVALERONE IC50 = 32.0 nM 7.50
CHEMBL553937 IC50 = 175.0 nM 6.76
CHEMBL558086 IC50 = 264.0 nM 6.58
CHEMBL894 BUPROPION IC50 = 1567.0 nM 5.80