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Assay Detail

CHEMBL1034822

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli DNA topoisomerase 4 subunit B ParE assessed as inorganic phosphate release by malachite green complex formation assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

DNA gyrase (GyrB)/topoisomerase IV (ParE) inhibitors: synthesis and antibacterial activity.
East SP, White CB, Barker O, Barker S, Bennett J, Brown D, Boyd EA, Brennan C, Chowdhury C, Collins I, Convers-Reignier E, Dymock BW, Fletcher R, Haydon DJ, Gardiner M, Hatcher S, Ingram P, Lancett P, Mortenson P, Papadopoulos K, Smee C, Thomaides-Brears HB, Tye H, Workman J, Czaplewski LG.
Bioorg Med Chem Lett (2009) 19 : 894 -899
PubMed 19095445 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 5.06 5.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL36506 NOVOBIOCIN 4.0 1 5.88
CHEMBL487851 1 5.46
CHEMBL473810 1 4.96
CHEMBL515259 1 4.60
CHEMBL474194 1 4.40
CHEMBL481498 1 -
CHEMBL446903 1 -
CHEMBL481497 1 -
CHEMBL475773 1 -
CHEMBL474605 1 -
CHEMBL474604 1 -
CHEMBL473423 1 -
CHEMBL481904 1 -
CHEMBL481903 1 -
CHEMBL481734 1 -
CHEMBL481733 1 -
CHEMBL454442 1 -
CHEMBL514638 1 -
CHEMBL475175 1 -
CHEMBL475174 1 -
CHEMBL475173 1 -
CHEMBL475172 1 -
CHEMBL474986 1 -
CHEMBL443886 1 -
CHEMBL473811 1 -
CHEMBL473644 1 -
CHEMBL503393 1 -
CHEMBL471970 1 -
CHEMBL443651 1 -
CHEMBL511662 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL36506 NOVOBIOCIN IC50 = 1320.0 nM 5.88
CHEMBL487851 IC50 = 3500.0 nM 5.46
CHEMBL473810 IC50 = 11000.0 nM 4.96
CHEMBL515259 IC50 = 25000.0 nM 4.60
CHEMBL474194 IC50 = 40000.0 nM 4.40
CHEMBL481498 IC50 - -
CHEMBL480736 IC50 = 145000.0 nM -
CHEMBL481497 IC50 - -
CHEMBL475773 IC50 - -
CHEMBL474605 IC50 - -
CHEMBL474604 IC50 - -
CHEMBL473423 IC50 - -
CHEMBL481904 IC50 - -
CHEMBL481903 IC50 - -
CHEMBL481734 IC50 - -
CHEMBL481733 IC50 - -
CHEMBL454442 IC50 - -
CHEMBL446903 IC50 - -
CHEMBL514638 IC50 - -
CHEMBL475175 IC50 - -
CHEMBL475174 IC50 - -
CHEMBL475173 IC50 - -
CHEMBL475172 IC50 - -
CHEMBL474986 IC50 - -
CHEMBL443886 IC50 - -
CHEMBL473811 IC50 - -
CHEMBL473644 IC50 - -
CHEMBL503393 IC50 - -
CHEMBL471970 IC50 - -
CHEMBL443651 IC50 - -
CHEMBL511662 IC50 - -