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Assay Detail

CHEMBL1034979

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Binding
Inhibition of human thymidylate synthase
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Thymidylate synthase (CHEMBL1952)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent dual thymidylate synthase and dihydrofolate reductase inhibitors: classical and nonclassical 2-amino-4-oxo-5-arylthio-substituted-6-methylthieno[2,3-d]pyrimidine antifolates.
Gangjee A, Qiu Y, Li W, Kisliuk RL.
J Med Chem (2008) 51 : 5789 -5797
PubMed 18800768 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.52 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL522363 1 7.40
CHEMBL225180 1 7.38
CHEMBL422395 1 7.07
CHEMBL492836 1 6.96
CHEMBL492838 1 6.96
CHEMBL495124 1 6.92
CHEMBL400485 1 6.89
CHEMBL400364 1 6.82
CHEMBL522517 1 6.58
CHEMBL495125 1 6.55
CHEMBL492839 1 6.00
CHEMBL449543 1 5.96
CHEMBL494067 1 5.92
CHEMBL492837 1 5.34
CHEMBL225072 PEMETREXED 4.0 1 5.02
CHEMBL34259 METHOTREXATE 4.0 1 -
CHEMBL22 TRIMETHOPRIM 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL522363 IC50 = 40.0 nM 7.40
CHEMBL225180 IC50 = 42.0 nM 7.38
CHEMBL422395 IC50 = 85.0 nM 7.07
CHEMBL492836 IC50 = 110.0 nM 6.96
CHEMBL492838 IC50 = 110.0 nM 6.96
CHEMBL495124 IC50 = 120.0 nM 6.92
CHEMBL400485 IC50 = 130.0 nM 6.89
CHEMBL400364 IC50 = 150.0 nM 6.82
CHEMBL522517 IC50 = 260.0 nM 6.58
CHEMBL495125 IC50 = 280.0 nM 6.55
CHEMBL492839 IC50 = 1000.0 nM 6.00
CHEMBL449543 IC50 = 1100.0 nM 5.96
CHEMBL494067 IC50 = 1200.0 nM 5.92
CHEMBL492837 IC50 = 4600.0 nM 5.34
CHEMBL225072 PEMETREXED IC50 = 9500.0 nM 5.02
CHEMBL34259 METHOTREXATE IC50 - -
CHEMBL22 TRIMETHOPRIM IC50 - -