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Assay Detail

CHEMBL1036476

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Binding
Inhibition of mPGES1 in IL1-beta treated human A549 cell microsomal membrane assessed as residual enzyme activity after 1 min by measuring PGE2 level using RP-HPLC method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Prostaglandin E synthase (CHEMBL5658)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Arylpyrrolizines as inhibitors of microsomal prostaglandin E2 synthase-1 (mPGES-1) or as dual inhibitors of mPGES-1 and 5-lipoxygenase (5-LOX).
Liedtke AJ, Keck PR, Lehmann F, Koeberle A, Werz O, Laufer SA.
J Med Chem (2009) 52 : 4968 -4972
PubMed 19719242 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.62 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL182032 1 7.92
CHEMBL550489 1 5.75
CHEMBL551236 1 5.68
CHEMBL29097 1 5.68
CHEMBL564318 1 5.41
CHEMBL563280 1 5.35
CHEMBL550894 1 5.32
CHEMBL560898 1 5.23
CHEMBL561824 1 5.21
CHEMBL300982 LICOFELONE 2.0 1 5.17
CHEMBL549341 1 5.14
CHEMBL294159 1 -
CHEMBL292239 1 -
CHEMBL565159 1 -
CHEMBL57062 1 -
CHEMBL560897 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL182032 IC50 = 12.0 nM 7.92
CHEMBL550489 IC50 = 1800.0 nM 5.75
CHEMBL551236 IC50 = 2100.0 nM 5.68
CHEMBL29097 IC50 = 2110.0 nM 5.68
CHEMBL564318 IC50 = 3900.0 nM 5.41
CHEMBL563280 IC50 = 4500.0 nM 5.35
CHEMBL550894 IC50 = 4800.0 nM 5.32
CHEMBL560898 IC50 = 5900.0 nM 5.23
CHEMBL561824 IC50 = 6200.0 nM 5.21
CHEMBL300982 LICOFELONE IC50 = 6700.0 nM 5.17
CHEMBL549341 IC50 = 7200.0 nM 5.14
CHEMBL294159 IC50 - -
CHEMBL292239 IC50 - -
CHEMBL565159 IC50 - -
CHEMBL57062 IC50 - -
CHEMBL560897 IC50 - -