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Assay Detail

CHEMBL1038010

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at histamine H1 receptor in human SK-N-SH cells assessed as histamine-induced maximum intracellular calcium spike at phase 1 treated 10 to 15 seconds before histamine challenge
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SK-N-SH
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological activity of 5-styryl and 5-phenethyl-substituted 2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indoles.
Ivachtchenko AV, Frolov EB, Mitkin OD, Tkachenko SE, Okun IM, Khvat AV.
Bioorg Med Chem Lett (2010) 20 : 78 -82
PubMed 19945877 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.20 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL592753 1 7.52
CHEMBL603049 1 7.51
CHEMBL589150 1 7.50
CHEMBL599913 1 7.48
CHEMBL591803 1 7.46
CHEMBL589148 1 7.40
CHEMBL592752 MARITUPIRDINE 2.0 1 7.40
CHEMBL589147 1 7.18
CHEMBL589388 1 7.17
CHEMBL605405 1 7.16
CHEMBL606036 1 7.01
CHEMBL589389 1 6.98
CHEMBL605081 1 6.95
CHEMBL589390 LATREPIRDINE 3.0 1 6.80
CHEMBL591568 1 6.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL592753 IC50 = 30.0 nM 7.52
CHEMBL603049 IC50 = 31.0 nM 7.51
CHEMBL589150 IC50 = 32.0 nM 7.50
CHEMBL599913 IC50 = 33.0 nM 7.48
CHEMBL591803 IC50 = 35.0 nM 7.46
CHEMBL589148 IC50 = 40.0 nM 7.40
CHEMBL592752 MARITUPIRDINE IC50 = 40.0 nM 7.40
CHEMBL589147 IC50 = 66.0 nM 7.18
CHEMBL589388 IC50 = 68.0 nM 7.17
CHEMBL605405 IC50 = 70.0 nM 7.16
CHEMBL606036 IC50 = 98.0 nM 7.01
CHEMBL589389 IC50 = 104.0 nM 6.98
CHEMBL605081 IC50 = 112.0 nM 6.95
CHEMBL589390 LATREPIRDINE IC50 = 158.0 nM 6.80
CHEMBL591568 IC50 = 345.0 nM 6.46