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Assay Detail

CHEMBL1038910

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of mouse DHFR by enzyme kinetics assay in presence of variable DHFA level
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL4564)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Design, synthesis, and X-ray crystal structures of 2,4-diaminofuro[2,3-d]pyrimidines as multireceptor tyrosine kinase and dihydrofolate reductase inhibitors.
Gangjee A, Li W, Lin L, Zeng Y, Ihnat M, Warnke LA, Green DW, Cody V, Pace J, Queener SF.
Bioorg Med Chem (2009) 17 : 7324 -7336
PubMed 19748785 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 6.58 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL595757 1 7.22
CHEMBL603198 1 7.22
CHEMBL606039 1 7.21
CHEMBL594810 1 7.16
CHEMBL603849 1 7.16
CHEMBL594320 1 7.10
CHEMBL595497 1 6.85
CHEMBL610181 1 6.80
CHEMBL22 TRIMETHOPRIM 4.0 1 6.30
CHEMBL604879 1 6.26
CHEMBL606362 1 6.21
CHEMBL594339 1 6.11
CHEMBL593154 1 6.04
CHEMBL605413 1 5.55
CHEMBL594790 1 5.48
CHEMBL34259 METHOTREXATE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL595757 Ki = 60.0 nM 7.22
CHEMBL603198 Ki = 60.0 nM 7.22
CHEMBL606039 Ki = 62.0 nM 7.21
CHEMBL594810 Ki = 70.0 nM 7.16
CHEMBL603849 Ki = 70.0 nM 7.16
CHEMBL594320 Ki = 80.0 nM 7.10
CHEMBL595497 Ki = 140.0 nM 6.85
CHEMBL610181 Ki = 160.0 nM 6.80
CHEMBL22 TRIMETHOPRIM Ki = 500.0 nM 6.30
CHEMBL604879 Ki = 550.0 nM 6.26
CHEMBL606362 Ki = 620.0 nM 6.21
CHEMBL594339 Ki = 780.0 nM 6.11
CHEMBL593154 Ki = 910.0 nM 6.04
CHEMBL605413 Ki = 2800.0 nM 5.55
CHEMBL594790 Ki = 3300.0 nM 5.48
CHEMBL34259 METHOTREXATE Ki = 0.003 nM -