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Assay Detail

CHEMBL1041602

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant PDE4D catalytic domain cloned from human HL60 cells assessed as inhibition of cAMP hydrolysis
19
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HL-60
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3',5'-cyclic-AMP phosphodiesterase 4D (CHEMBL288)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of novel phthalazinone derivatives as topically active phosphodiesterase 4 inhibitors.
Kagayama K, Morimoto T, Nagata S, Katoh F, Zhang X, Inoue N, Hashino A, Kageyama K, Shikaura J, Niwa T.
Bioorg Med Chem (2009) 17 : 6959 -6970
PubMed 19744860 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 8.49 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL593656 1 9.52
CHEMBL594108 1 9.40
CHEMBL594109 1 9.15
CHEMBL595064 1 9.05
CHEMBL593877 2 9.05
CHEMBL595272 1 9.00
CHEMBL596447 1 8.89
CHEMBL592931 1 8.89
CHEMBL596394 1 8.85
CHEMBL595038 1 8.70
CHEMBL605096 1 8.37
CHEMBL595959 1 8.28
CHEMBL603626 1 8.09
CHEMBL595039 1 8.01
CHEMBL605935 1 7.84
CHEMBL603194 1 7.79
CHEMBL592942 1 7.34
CHEMBL440878 1 7.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL593656 IC50 = 0.3 nM 9.52
CHEMBL594108 IC50 = 0.4 nM 9.40
CHEMBL594109 IC50 = 0.7 nM 9.15
CHEMBL595064 IC50 = 0.9 nM 9.05
CHEMBL593877 IC50 = 0.9 nM 9.05
CHEMBL595272 IC50 = 1.0 nM 9.00
CHEMBL596447 IC50 = 1.3 nM 8.89
CHEMBL592931 IC50 = 1.3 nM 8.89
CHEMBL596394 IC50 = 1.4 nM 8.85
CHEMBL595038 IC50 = 2.0 nM 8.70
CHEMBL605096 IC50 = 4.3 nM 8.37
CHEMBL595959 IC50 = 5.2 nM 8.28
CHEMBL603626 IC50 = 8.2 nM 8.09
CHEMBL593877 IC50 = 8.8 nM 8.06
CHEMBL595039 IC50 = 9.7 nM 8.01
CHEMBL605935 IC50 = 14.3 nM 7.84
CHEMBL603194 IC50 = 16.4 nM 7.79
CHEMBL592942 IC50 = 45.9 nM 7.34
CHEMBL440878 IC50 = 85.0 nM 7.07