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Assay Detail

CHEMBL1042962

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of human glucokinase expressed in Escherichia coli BL21(DE3) by G6PDH-coupled spectrometry
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hexokinase-4 (CHEMBL3820)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Benzamide derivatives as dual-action hypoglycemic agents that inhibit glycogen phosphorylase and activate glucokinase.
Zhang L, Li H, Zhu Q, Liu J, Chen L, Leng Y, Jiang H, Liu H.
Bioorg Med Chem (2009) 17 : 7301 -7312
PubMed 19758809 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 5.82 7.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL608393 1 7.17
CHEMBL391800 1 5.87
CHEMBL239692 1 5.73
CHEMBL239481 1 5.67
CHEMBL394757 1 5.60
CHEMBL394709 1 4.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL608393 EC50 = 67.11 nM 7.17
CHEMBL391800 EC50 = 1360.0 nM 5.87
CHEMBL239692 EC50 = 1870.0 nM 5.73
CHEMBL239481 EC50 = 2130.0 nM 5.67
CHEMBL394757 EC50 = 2530.0 nM 5.60
CHEMBL394709 EC50 = 12560.0 nM 4.90