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Assay Detail

CHEMBL1043646

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [35S]-labeled ligand from VLA4 in human Jurkat cells washed with buffer containing non activating Ca2+ and Mg2+ by competitive binding assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Jurkat
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Integrin alpha-4/beta-1 (CHEMBL1907599)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of N-{N-[(3-cyanobenzene) sulfonyl]-4(R)-(3,3-difluoropiperidin-1-yl)-(l)-prolyl}-4-[(3',5'-dichloro-isonicotinoyl) amino]-(l)-phenylalanine (MK-0617), a highly potent and orally active VLA-4 antagonist.
Venkatraman S, Lebsack AD, Alves K, Gardner MF, James J, Lingham RB, Maniar S, Mumford RA, Si Q, Stock N, Treonze KM, Wang B, Zunic J, Munoz B.
Bioorg Med Chem Lett (2009) 19 : 5803 -5806
PubMed 19713111 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 9.82 10.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL569442 1 10.70
CHEMBL566154 1 10.52
CHEMBL569443 1 10.00
CHEMBL569263 1 9.96
CHEMBL569276 1 9.92
CHEMBL506044 1 9.82
CHEMBL569403 1 9.82
CHEMBL567433 1 9.68
CHEMBL569255 1 9.66
CHEMBL569444 1 9.66
CHEMBL569302 1 9.62
CHEMBL571805 1 9.57
CHEMBL565499 1 9.46
CHEMBL569407 1 9.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL569442 IC50 = 0.02 nM 10.70
CHEMBL566154 IC50 = 0.03 nM 10.52
CHEMBL569443 IC50 = 0.1 nM 10.00
CHEMBL569263 IC50 = 0.11 nM 9.96
CHEMBL569276 IC50 = 0.12 nM 9.92
CHEMBL506044 IC50 = 0.15 nM 9.82
CHEMBL569403 IC50 = 0.15 nM 9.82
CHEMBL567433 IC50 = 0.21 nM 9.68
CHEMBL569255 IC50 = 0.22 nM 9.66
CHEMBL569444 IC50 = 0.22 nM 9.66
CHEMBL569302 IC50 = 0.24 nM 9.62
CHEMBL571805 IC50 = 0.27 nM 9.57
CHEMBL565499 IC50 = 0.35 nM 9.46
CHEMBL569407 IC50 = 0.9 nM 9.05