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Assay Detail

CHEMBL1043647

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Receptor occupancy of VLA4 in whole blood washed with buffer containing non activating Ca2+ and Mg2+ at 37 degC after 3 hrs
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Blood
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Integrin alpha-4/beta-1 (CHEMBL1907599)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of N-{N-[(3-cyanobenzene) sulfonyl]-4(R)-(3,3-difluoropiperidin-1-yl)-(l)-prolyl}-4-[(3',5'-dichloro-isonicotinoyl) amino]-(l)-phenylalanine (MK-0617), a highly potent and orally active VLA-4 antagonist.
Venkatraman S, Lebsack AD, Alves K, Gardner MF, James J, Lingham RB, Maniar S, Mumford RA, Si Q, Stock N, Treonze KM, Wang B, Zunic J, Munoz B.
Bioorg Med Chem Lett (2009) 19 : 5803 -5806
PubMed 19713111 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.18 7.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL569444 1 7.43
CHEMBL565499 1 7.26
CHEMBL569403 1 7.25
CHEMBL571805 1 7.22
CHEMBL506044 1 7.19
CHEMBL569407 1 7.19
CHEMBL569255 1 7.19
CHEMBL567433 1 7.16
CHEMBL569302 1 7.12
CHEMBL569442 1 7.10
CHEMBL569276 1 7.10
CHEMBL569263 1 7.10
CHEMBL569443 1 7.09
CHEMBL566154 1 7.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL569444 IC50 = 37.0 nM 7.43
CHEMBL565499 IC50 = 55.0 nM 7.26
CHEMBL569403 IC50 = 56.0 nM 7.25
CHEMBL571805 IC50 = 60.0 nM 7.22
CHEMBL506044 IC50 = 64.0 nM 7.19
CHEMBL569407 IC50 = 65.0 nM 7.19
CHEMBL569255 IC50 = 65.0 nM 7.19
CHEMBL567433 IC50 = 70.0 nM 7.16
CHEMBL569302 IC50 = 75.0 nM 7.12
CHEMBL569442 IC50 = 80.0 nM 7.10
CHEMBL569276 IC50 = 80.0 nM 7.10
CHEMBL569263 IC50 = 80.0 nM 7.10
CHEMBL569443 IC50 = 81.0 nM 7.09
CHEMBL566154 IC50 = 90.0 nM 7.05