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Assay Detail

CHEMBL1046105

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CLR expressed in HEK293 cells coexpressing human RAMP1 assessed as inhibition of human CGRPalpha-induced cAMP production after 5 mins by scintillation proximity assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Calcitonin gene-related peptide type 1 receptor (CHEMBL3798)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of azepanone calcitonin gene-related peptide (CGRP) receptor antagonists: development of novel spiropiperidines.
Burgey CS, Potteiger CM, Deng JZ, Mosser SD, Salvatore CA, Yu S, Roller S, Kane SA, Vacca JP, Williams TM.
Bioorg Med Chem Lett (2009) 19 : 6368 -6372
PubMed 19818613 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.73 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL236593 TELCAGEPANT 3.0 1 8.70
CHEMBL570746 1 8.57
CHEMBL571203 1 8.48
CHEMBL570531 1 7.96
CHEMBL570748 1 7.89
CHEMBL577110 1 7.64
CHEMBL570793 1 7.36
CHEMBL572114 1 7.26
CHEMBL571459 1 7.22
CHEMBL577957 1 7.03
CHEMBL577320 1 6.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL236593 TELCAGEPANT IC50 = 2.0 nM 8.70
CHEMBL570746 IC50 = 2.7 nM 8.57
CHEMBL571203 IC50 = 3.3 nM 8.48
CHEMBL570531 IC50 = 11.0 nM 7.96
CHEMBL570748 IC50 = 13.0 nM 7.89
CHEMBL577110 IC50 = 23.0 nM 7.64
CHEMBL570793 IC50 = 44.0 nM 7.36
CHEMBL572114 IC50 = 55.0 nM 7.26
CHEMBL571459 IC50 = 60.0 nM 7.22
CHEMBL577957 IC50 = 94.0 nM 7.03
CHEMBL577320 IC50 = 120.0 nM 6.92