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Assay Detail

CHEMBL1049504

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal hexahistidine tagged HCV 1b NS5B polymerase expressed in baculovirus infected Sf21 cells after 90 mins by SPA
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 1b
Cell Type Sf21
Confidence 1 — Organism
Curated By Autocuration

Target

Hepatitis C virus (CHEMBL379)
Type ORGANISM
Organism Hepacivirus hominis

Publication

Discovery of benzimidazole-diamide finger loop (Thumb Pocket I) allosteric inhibitors of HCV NS5B polymerase: Implementing parallel synthesis for rapid linker optimization.
Goulet S, Poupart MA, Gillard J, Poirier M, Kukolj G, Beaulieu PL.
Bioorg Med Chem Lett (2010) 20 : 196 -200
PubMed 19945870 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.45 7.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL366574 1 7.72
CHEMBL590373 1 7.22
CHEMBL605616 1 7.05
CHEMBL594318 1 7.00
CHEMBL590850 1 6.89
CHEMBL590851 1 6.55
CHEMBL376487 1 6.52
CHEMBL591089 1 6.47
CHEMBL603143 1 6.44
CHEMBL591560 1 6.41
CHEMBL601480 1 6.40
CHEMBL594592 1 6.35
CHEMBL592027 1 6.05
CHEMBL591090 1 6.05
CHEMBL591792 1 5.82
CHEMBL590848 1 5.79
CHEMBL264034 1 4.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL366574 IC50 = 19.0 nM 7.72
CHEMBL590373 IC50 = 60.0 nM 7.22
CHEMBL605616 IC50 = 90.0 nM 7.05
CHEMBL594318 IC50 = 100.0 nM 7.00
CHEMBL590850 IC50 = 130.0 nM 6.89
CHEMBL590851 IC50 = 280.0 nM 6.55
CHEMBL376487 IC50 = 300.0 nM 6.52
CHEMBL591089 IC50 = 340.0 nM 6.47
CHEMBL603143 IC50 = 360.0 nM 6.44
CHEMBL591560 IC50 = 390.0 nM 6.41
CHEMBL601480 IC50 = 400.0 nM 6.40
CHEMBL594592 IC50 = 450.0 nM 6.35
CHEMBL592027 IC50 = 900.0 nM 6.05
CHEMBL591090 IC50 = 900.0 nM 6.05
CHEMBL591792 IC50 = 1520.0 nM 5.82
CHEMBL590848 IC50 = 1610.0 nM 5.79
CHEMBL264034 IC50 = 14000.0 nM 4.85