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Assay Detail

CHEMBL1058716

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of pig cerebral cortex Na+/K+-ATPase by colorimetric microplate-based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Tissue Cerebral cortex
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Cardenolides from Pergularia tomentosa display cytotoxic activity resulting from their potent inhibition of Na+/K+-ATPase.
Piacente S, Masullo M, De Nève N, Dewelle J, Hamed A, Kiss R, Mijatovic T.
J Nat Prod (2009) 72 : 1087 -1091
PubMed 19555122 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.22 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL499134 GHALAKINOSIDE 1 6.52
CHEMBL447312 1 6.40
CHEMBL502127 CALACTIN 1 6.40
CHEMBL559102 1 6.30
CHEMBL555674 1 6.30
CHEMBL469426 1 6.16
CHEMBL558544 1 5.92
CHEMBL556108 1 5.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL499134 GHALAKINOSIDE IC50 = 300.0 nM 6.52
CHEMBL447312 IC50 = 400.0 nM 6.40
CHEMBL502127 CALACTIN IC50 = 400.0 nM 6.40
CHEMBL559102 IC50 = 500.0 nM 6.30
CHEMBL555674 IC50 = 500.0 nM 6.30
CHEMBL469426 IC50 = 700.0 nM 6.16
CHEMBL558544 IC50 = 1200.0 nM 5.92
CHEMBL556108 IC50 = 1700.0 nM 5.77