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Assay Detail

CHEMBL1060869

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CLR expressed in HEK293 cells coexpressing human RAMP1 assessed as inhibition of human CGRPalpha-induced cAMP production after 30 mins by scintillation proximity assay in presence of 50% human serum
11
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Calcitonin gene-related peptide type 1 receptor (CHEMBL3798)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The identification of potent, orally bioavailable tricyclic CGRP receptor antagonists.
Bell IM, Bednar RA, Corcoran HA, Fay JF, Gallicchio SN, Johnston VK, Hershey JC, Miller-Stein CM, Moore EL, Mosser SD, Roller SA, Salvatore CA, Theberge CR, Wong BK, Blair Zartman C, Kane SA, Williams TM, Graham SL, Vacca JP.
Bioorg Med Chem Lett (2009) 19 : 4740 -4742
PubMed 19577468 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.83 9.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL512773 1 9.21
CHEMBL562069 1 8.70
CHEMBL564487 ISOMER A 1 8.19
CHEMBL564088 EPIMER A 2 8.09
CHEMBL236593 TELCAGEPANT 3.0 1 7.96
CHEMBL1162991 1 7.89
CHEMBL549503 ISOMER A 1 7.64
CHEMBL564466 1 7.43
CHEMBL563727 1 7.17
CHEMBL556703 1 6.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL512773 IC50 = 0.62 nM 9.21
CHEMBL562069 IC50 = 2.0 nM 8.70
CHEMBL564487 ISOMER A IC50 = 6.5 nM 8.19
CHEMBL564088 EPIMER A IC50 = 8.1 nM 8.09
CHEMBL236593 TELCAGEPANT IC50 = 11.0 nM 7.96
CHEMBL1162991 IC50 = 13.0 nM 7.89
CHEMBL549503 ISOMER A IC50 = 23.0 nM 7.64
CHEMBL564088 EPIMER A IC50 = 24.0 nM 7.62
CHEMBL564466 IC50 = 37.0 nM 7.43
CHEMBL563727 IC50 = 67.0 nM 7.17
CHEMBL556703 IC50 = 530.0 nM 6.28