Assay Detail
Functional
CHEMBL1063015
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Inhibition of protein synthesis in human HeLa cells assessed as [35S]methionine incorporation into protein treated for 45 mins before [35S]methionine addition by Whatman paper analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HeLa |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Quassinoid inhibition of AP-1 function does not correlate with cytotoxicity or protein synthesis inhibition.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 7.13 | 8.47 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL487194 | AILANTHINONE | — | 1 | 8.47 |
| CHEMBL509895 | BRUCEANTIN | — | 1 | 7.29 |
| CHEMBL458347 | GLAUCARUBINONE | — | 1 | 6.57 |
| CHEMBL564009 | — | — | 1 | 6.17 |
| CHEMBL450464 | BRUSATOL | — | 1 | - |
| CHEMBL517016 | QUASSIN | — | 1 | - |
| CHEMBL472650 | GLAUCARUBOL | — | 1 | - |
| CHEMBL521373 | SERGEOLIDE | — | 1 | - |
| CHEMBL519243 | HOLACANTHONE | — | 1 | - |
| CHEMBL558152 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL487194 | AILANTHINONE | IC50 | = | 3.4 | nM | 8.47 |
| CHEMBL509895 | BRUCEANTIN | IC50 | = | 51.0 | nM | 7.29 |
| CHEMBL458347 | GLAUCARUBINONE | IC50 | = | 270.0 | nM | 6.57 |
| CHEMBL564009 | — | IC50 | = | 670.0 | nM | 6.17 |
| CHEMBL450464 | BRUSATOL | IC50 | < | 1000.0 | nM | - |
| CHEMBL517016 | QUASSIN | IC50 | > | 2000.0 | nM | - |
| CHEMBL472650 | GLAUCARUBOL | IC50 | > | 2000.0 | nM | - |
| CHEMBL521373 | SERGEOLIDE | IC50 | < | 1000.0 | nM | - |
| CHEMBL519243 | HOLACANTHONE | IC50 | - | — | - | |
| CHEMBL558152 | — | IC50 | > | 2000.0 | nM | - |