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Assay Detail

CHEMBL1065651

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]granisetron from human 5HT3A receptor expressed in HEK293 cells by scintillation counting
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 3A (CHEMBL1899)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Toward biophysical probes for the 5-HT3 receptor: structure-activity relationship study of granisetron derivatives.
Vernekar SK, Hallaq HY, Clarkson G, Thompson AJ, Silvestri L, Lummis SC, Lochner M.
J Med Chem (2010) 53 : 2324 -2328
PubMed 20146481 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 7.52 9.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL596951 1 9.77
CHEMBL605772 1 9.64
CHEMBL599011 1 9.17
CHEMBL289469 GRANISETRON 4.0 1 8.84
CHEMBL2309589 1 8.72
CHEMBL598804 1 8.14
CHEMBL596952 1 7.58
CHEMBL597984 1 7.23
CHEMBL599012 1 7.15
CHEMBL596758 1 6.62
CHEMBL603673 1 6.55
CHEMBL598803 1 6.43
CHEMBL604083 1 6.13
CHEMBL598805 1 5.52
CHEMBL605562 1 5.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL596951 Ki = 0.17 nM 9.77
CHEMBL605772 Ki = 0.23 nM 9.64
CHEMBL599011 Ki = 0.67 nM 9.17
CHEMBL289469 GRANISETRON Ki = 1.45 nM 8.84
CHEMBL2309589 Ki = 1.89 nM 8.72
CHEMBL598804 Ki = 7.3 nM 8.14
CHEMBL596952 Ki = 26.3 nM 7.58
CHEMBL597984 Ki = 59.3 nM 7.23
CHEMBL599012 Ki = 71.1 nM 7.15
CHEMBL596758 Ki = 237.0 nM 6.62
CHEMBL603673 Ki = 279.0 nM 6.55
CHEMBL598803 Ki = 375.0 nM 6.43
CHEMBL604083 Ki = 749.0 nM 6.13
CHEMBL598805 Ki = 3029.0 nM 5.52
CHEMBL605562 Ki = 5306.0 nM 5.28