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Assay Detail

CHEMBL1067826

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human recombinant 5HT2C receptor expressed in HEK293E cells by FLIPR assay
17
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293E
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2C (CHEMBL225)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tricyclic dihydroquinazolinones as novel 5-HT2C selective and orally efficacious anti-obesity agents.
Ahmad S, Ngu K, Miller KJ, Wu G, Hung CP, Malmstrom S, Zhang G, O'Tanyi E, Keim WJ, Cullen MJ, Rohrbach KW, Thomas M, Ung T, Qu Q, Gan J, Narayanan R, Pelleymounter MA, Robl JA.
Bioorg Med Chem Lett (2010) 20 : 1128 -1133
PubMed 20022752 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 7.31 8.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL590483 1 8.74
CHEMBL590580 1 8.70
CHEMBL590531 2 8.18
CHEMBL478 CHLOROPHENYLPIPERAZINE 2.0 1 7.82
CHEMBL590816 1 7.80
CHEMBL590331 1 7.47
CHEMBL605402 1 7.37
CHEMBL590078 2 7.35
CHEMBL590333 1 7.04
CHEMBL590482 1 6.96
CHEMBL590089 1 6.88
CHEMBL590077 1 6.86
CHEMBL590332 1 5.38
CHEMBL603527 1 -
CHEMBL590721 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL590483 EC50 = 1.8 nM 8.74
CHEMBL590580 EC50 = 2.0 nM 8.70
CHEMBL590531 EC50 = 6.6 nM 8.18
CHEMBL478 CHLOROPHENYLPIPERAZINE EC50 = 15.0 nM 7.82
CHEMBL590816 EC50 = 16.0 nM 7.80
CHEMBL590331 EC50 = 34.0 nM 7.47
CHEMBL605402 EC50 = 43.0 nM 7.37
CHEMBL590078 EC50 = 45.0 nM 7.35
CHEMBL590333 EC50 = 92.0 nM 7.04
CHEMBL590531 EC50 = 92.0 nM 7.04
CHEMBL590482 EC50 = 110.0 nM 6.96
CHEMBL590089 EC50 = 133.0 nM 6.88
CHEMBL590077 EC50 = 137.0 nM 6.86
CHEMBL590078 EC50 = 989.0 nM 6.00
CHEMBL590332 EC50 = 4197.0 nM 5.38
CHEMBL603527 EC50 > 10000.0 nM -
CHEMBL590721 EC50 - -