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Assay Detail

CHEMBL1074807

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human full length PPARgamma expressed in human HepG2 cells co-transfected with PPRE3-TK-luc assessed as induction of beta-galactosidase activity by firefly luciferase assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of novel bis-oximinoalkanoic acids as potent PPAR alpha agonists.
Pingali H, Jain M, Shah S, Zaware P, Makadia P, Pola S, Thube B, Patel D, Patil P, Priyadarshini P, Suthar D, Shah M, Giri S, Patel P.
Bioorg Med Chem Lett (2010) 20 : 1156 -1161
PubMed 20022492 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 6.33 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL592054 IMIGLITAZAR 3.0 1 8.40
CHEMBL591348 1 7.00
CHEMBL591347 1 6.70
CHEMBL589256 1 6.70
CHEMBL592234 1 6.70
CHEMBL589747 1 6.16
CHEMBL592235 1 6.10
CHEMBL592236 1 6.00
CHEMBL591533 1 5.96
CHEMBL591055 1 5.85
CHEMBL591534 1 5.85
CHEMBL603612 1 5.80
CHEMBL591539 1 5.72
CHEMBL606462 1 5.68
CHEMBL590341 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL592054 IMIGLITAZAR EC50 = 4.0 nM 8.40
CHEMBL591348 EC50 = 100.0 nM 7.00
CHEMBL591347 EC50 = 200.0 nM 6.70
CHEMBL589256 EC50 = 200.0 nM 6.70
CHEMBL592234 EC50 = 200.0 nM 6.70
CHEMBL589747 EC50 = 700.0 nM 6.16
CHEMBL592235 EC50 = 800.0 nM 6.10
CHEMBL592236 EC50 = 1000.0 nM 6.00
CHEMBL591533 EC50 = 1100.0 nM 5.96
CHEMBL591055 EC50 = 1400.0 nM 5.85
CHEMBL591534 EC50 = 1400.0 nM 5.85
CHEMBL603612 EC50 = 1600.0 nM 5.80
CHEMBL591539 EC50 = 1900.0 nM 5.72
CHEMBL606462 EC50 = 2100.0 nM 5.68
CHEMBL590341 EC50 - -