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Assay Detail

CHEMBL1100183

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human HT-29 cells after 72 hrs by FMCA method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Autocuration

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Cytotoxic effects of cardiac glycosides in colon cancer cells, alone and in combination with standard chemotherapeutic drugs.
Felth J, Rickardson L, Rosén J, Wickström M, Fryknäs M, Lindskog M, Bohlin L, Gullbo J.
J Nat Prod (2009) 72 : 1969 -1974
PubMed 19894733 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.90 6.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4285883 OLEANDRIN 1.0 1 6.36
CHEMBL399336 CONVALLATOXIN 1 6.26
CHEMBL600325 PROSCILLARIDIN 2.0 1 6.26
CHEMBL1751 DIGOXIN 4.0 1 5.85
CHEMBL254219 DIGITOXIN 4.0 1 5.39
CHEMBL404811 DIGITONIN 1 5.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4285883 OLEANDRIN IC50 = 440.0 nM 6.36
CHEMBL399336 CONVALLATOXIN IC50 = 550.0 nM 6.26
CHEMBL600325 PROSCILLARIDIN IC50 = 550.0 nM 6.26
CHEMBL1751 DIGOXIN IC50 = 1400.0 nM 5.85
CHEMBL254219 DIGITOXIN IC50 = 4070.0 nM 5.39
CHEMBL404811 DIGITONIN IC50 = 5600.0 nM 5.25