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Assay Detail

CHEMBL1100889

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of catalytic activity of human aminopeptidase N transfected in HEK293 cells assessed as formation of p-nitroaniline
23
Total Activities
12
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aminopeptidase N (CHEMBL1907)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The replacement of His(4) in angiotensin IV by conformationally constrained residues provides highly potent and selective analogues.
Lukaszuk A, Demaegdt H, Feytens D, Vanderheyden P, Vauquelin G, Tourwé D.
J Med Chem (2009) 52 : 5612 -5618
PubMed 19757839 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 7.18 8.07
Activity 11 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1077583 2 8.07
CHEMBL1077582 2 7.90
CHEMBL259019 2 7.56
CHEMBL1077592 2 7.30
CHEMBL1077586 2 7.28
CHEMBL1077585 2 7.26
CHEMBL261120 ANGIOTENSIN IV 1 7.25
CHEMBL260622 2 7.00
CHEMBL1077584 2 6.89
CHEMBL1077591 2 6.74
CHEMBL1077590 2 6.66
CHEMBL1077589 2 6.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1077583 Ki = 8.511 nM 8.07
CHEMBL1077582 Ki = 12.59 nM 7.90
CHEMBL259019 Ki = 27.54 nM 7.56
CHEMBL1077592 Ki = 50.12 nM 7.30
CHEMBL1077586 Ki = 52.48 nM 7.28
CHEMBL1077585 Ki = 54.95 nM 7.26
CHEMBL261120 ANGIOTENSIN IV Ki = 56.23 nM 7.25
CHEMBL260622 Ki = 100.0 nM 7.00
CHEMBL1077584 Ki = 128.82 nM 6.89
CHEMBL1077591 Ki = 181.97 nM 6.74
CHEMBL1077590 Ki = 218.78 nM 6.66
CHEMBL1077589 Ki = 512.86 nM 6.29
CHEMBL1077589 Activity - -
CHEMBL1077590 Activity - -
CHEMBL1077591 Activity - -
CHEMBL1077592 Activity - -
CHEMBL1077582 Activity - -
CHEMBL1077583 Activity - -
CHEMBL1077584 Activity - -
CHEMBL1077585 Activity - -
CHEMBL1077586 Activity - -
CHEMBL259019 Activity - -
CHEMBL260622 Activity - -