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Assay Detail

CHEMBL1101545

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of SHH in mouse Shh Light2 cells after 30 hrs by GLI-responsive bright glo luciferase reporter gene assay
27
Total Activities
14
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type Shh Light II
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sonic hedgehog protein (CHEMBL5387)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Syntheses of aminoalcohol-derived macrocycles leading to a small-molecule binder to and inhibitor of Sonic Hedgehog.
Peng LF, Stanton BZ, Maloof N, Wang X, Schreiber SL.
Bioorg Med Chem Lett (2009) 19 : 6319 -6325
PubMed 19819139 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
ECmax 14 - -
EC50 13 4.75 5.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL473392 ROBOTNIKININ 2 5.40
CHEMBL1087150 2 5.40
CHEMBL1087530 2 5.16
CHEMBL1081556 2 5.00
CHEMBL1080988 2 4.82
CHEMBL1079558 2 4.82
CHEMBL1088172 2 4.82
CHEMBL1080649 2 4.70
CHEMBL1080987 2 4.52
CHEMBL1076336 2 4.51
CHEMBL1080091 2 4.22
CHEMBL1079557 2 4.22
CHEMBL1076352 2 4.16
CHEMBL1087151 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL473392 ROBOTNIKININ EC50 = 4000.0 nM 5.40
CHEMBL1087150 EC50 = 4000.0 nM 5.40
CHEMBL1087530 EC50 = 7000.0 nM 5.16
CHEMBL1081556 EC50 = 10000.0 nM 5.00
CHEMBL1080988 EC50 = 15000.0 nM 4.82
CHEMBL1079558 EC50 = 15000.0 nM 4.82
CHEMBL1088172 EC50 = 15000.0 nM 4.82
CHEMBL1080649 EC50 = 20000.0 nM 4.70
CHEMBL1080987 EC50 = 30000.0 nM 4.52
CHEMBL1076336 EC50 = 31000.0 nM 4.51
CHEMBL1079557 EC50 = 60000.0 nM 4.22
CHEMBL1080091 EC50 = 60000.0 nM 4.22
CHEMBL1076352 EC50 = 70000.0 nM 4.16
CHEMBL1080988 ECmax = 125.0 uM -
CHEMBL1076336 ECmax = 125.0 uM -
CHEMBL1080987 ECmax = 125.0 uM -
CHEMBL1080091 ECmax = 125.0 uM -
CHEMBL1076352 ECmax = 125.0 uM -
CHEMBL1079557 ECmax = 125.0 uM -
CHEMBL1079558 ECmax = 125.0 uM -
CHEMBL1081556 ECmax = 15.6 uM -
CHEMBL1080649 ECmax = 125.0 uM -
CHEMBL473392 ROBOTNIKININ ECmax = 62.5 uM -
CHEMBL1088172 ECmax = 125.0 uM -
CHEMBL1087530 ECmax = 62.5 uM -
CHEMBL1087150 ECmax = 15.6 uM -
CHEMBL1087151 ECmax = 125.0 uM -