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Assay Detail

CHEMBL1102983

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant GSK3-beta assessed as [gamma33]ATP transfer to biotinylated CREB-peptide substrate after 1 hr by scintillation counting
32
Total Activities
31
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent and bioavailable GSK-3beta inhibitors.
Gong L, Hirschfeld D, Tan YC, Heather Hogg J, Peltz G, Avnur Z, Dunten P.
Bioorg Med Chem Lett (2010) 20 : 1693 -1696
PubMed 20138512 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 7.55 9.40
Kd 1 10.28 10.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1082152 2 10.28
CHEMBL1082153 1 9.40
CHEMBL1080901 1 9.25
CHEMBL1082151 1 9.00
CHEMBL54547 1 8.74
CHEMBL1081973 1 8.74
CHEMBL1088149 1 8.62
CHEMBL1081294 1 8.49
CHEMBL431933 1 8.44
CHEMBL1081617 1 8.19
CHEMBL1087242 1 8.18
CHEMBL1087499 1 8.15
CHEMBL1081972 1 8.09
CHEMBL1088724 1 8.00
CHEMBL26501 RO-320432 1 7.70
CHEMBL1087243 1 7.66
CHEMBL53745 1 7.66
CHEMBL1081616 1 7.51
CHEMBL1081128 1 7.46
CHEMBL1087380 1 7.38
CHEMBL322970 1 7.11
CHEMBL293542 1 7.05
CHEMBL430574 PAULLONE 1 7.00
CHEMBL1080933 1 6.66
CHEMBL1086734 1 6.46
CHEMBL1086732 1 6.14
CHEMBL1081293 1 5.89
CHEMBL1086733 1 5.70
CHEMBL1081260 1 5.55
CHEMBL1081261 1 5.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1082152 Kd = 0.053 nM 10.28
CHEMBL1082153 IC50 = 0.4 nM 9.40
CHEMBL1080901 IC50 = 0.56 nM 9.25
CHEMBL1082152 IC50 = 0.6 nM 9.22
CHEMBL1082151 IC50 = 1.0 nM 9.00
CHEMBL54547 IC50 = 1.8 nM 8.74
CHEMBL1081973 IC50 = 1.8 nM 8.74
CHEMBL1088149 IC50 = 2.4 nM 8.62
CHEMBL1081294 IC50 = 3.2 nM 8.49
CHEMBL431933 IC50 = 3.6 nM 8.44
CHEMBL1081617 IC50 = 6.4 nM 8.19
CHEMBL1087242 IC50 = 6.6 nM 8.18
CHEMBL1087499 IC50 = 7.0 nM 8.15
CHEMBL1081972 IC50 = 8.2 nM 8.09
CHEMBL1088724 IC50 = 10.0 nM 8.00
CHEMBL26501 RO-320432 IC50 = 20.0 nM 7.70
CHEMBL53745 IC50 = 22.0 nM 7.66
CHEMBL1087243 IC50 = 22.0 nM 7.66
CHEMBL1081616 IC50 = 31.0 nM 7.51
CHEMBL1081128 IC50 = 35.0 nM 7.46
CHEMBL1087380 IC50 = 42.0 nM 7.38
CHEMBL322970 IC50 = 77.0 nM 7.11
CHEMBL293542 IC50 = 90.0 nM 7.05
CHEMBL430574 PAULLONE IC50 = 100.0 nM 7.00
CHEMBL1080933 IC50 = 220.0 nM 6.66
CHEMBL1086734 IC50 = 350.0 nM 6.46
CHEMBL1086732 IC50 = 730.0 nM 6.14
CHEMBL1081293 IC50 = 1300.0 nM 5.89
CHEMBL1086733 IC50 = 2000.0 nM 5.70
CHEMBL1081260 IC50 = 2800.0 nM 5.55
CHEMBL1081261 IC50 = 3100.0 nM 5.51
CHEMBL1080932 IC50 = 8200.0 nM 5.09