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Assay Detail

CHEMBL1103719

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GST-tagged-PDK1 expressed in HEK293 cells
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3-phosphoinositide-dependent protein kinase 1 (CHEMBL2534)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The identification of 8,9-dimethoxy-5-(2-aminoalkoxy-pyridin-3-yl)-benzo[c][2,7]naphthyridin-4-ylamines as potent inhibitors of 3-phosphoinositide-dependent kinase-1 (PDK-1).
Nittoli T, Dushin RG, Ingalls C, Cheung K, Floyd MB, Fraser H, Olland A, Hu Y, Grosu G, Han X, Arndt K, Guo B, Wissner A.
Eur J Med Chem (2010) 45 : 1379 -1386
PubMed 20074837 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 8.04 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1094320 1 8.52
CHEMBL1095621 1 8.52
CHEMBL1097955 1 8.52
CHEMBL1095278 1 8.30
CHEMBL1095279 1 8.22
CHEMBL1097956 1 8.22
CHEMBL1097972 1 8.22
CHEMBL1095612 1 8.22
CHEMBL1095931 1 8.10
CHEMBL1096271 1 8.10
CHEMBL1097602 1 8.10
CHEMBL1097973 1 8.05
CHEMBL1098302 1 7.89
CHEMBL1094321 1 7.85
CHEMBL1095622 1 7.82
CHEMBL1097604 1 7.82
CHEMBL1095294 1 7.77
CHEMBL1094637 1 7.75
CHEMBL1095285 1 7.68
CHEMBL235157 1 7.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1094320 IC50 = 3.0 nM 8.52
CHEMBL1095621 IC50 = 3.0 nM 8.52
CHEMBL1097955 IC50 = 3.0 nM 8.52
CHEMBL1095278 IC50 = 5.0 nM 8.30
CHEMBL1095279 IC50 = 6.0 nM 8.22
CHEMBL1097956 IC50 = 6.0 nM 8.22
CHEMBL1097972 IC50 = 6.0 nM 8.22
CHEMBL1095612 IC50 = 6.0 nM 8.22
CHEMBL1095931 IC50 = 8.0 nM 8.10
CHEMBL1096271 IC50 = 8.0 nM 8.10
CHEMBL1097602 IC50 = 8.0 nM 8.10
CHEMBL1097973 IC50 = 9.0 nM 8.05
CHEMBL1098302 IC50 = 13.0 nM 7.89
CHEMBL1094321 IC50 = 14.0 nM 7.85
CHEMBL1095622 IC50 = 15.0 nM 7.82
CHEMBL1097604 IC50 = 15.0 nM 7.82
CHEMBL1095294 IC50 = 17.0 nM 7.77
CHEMBL1094637 IC50 = 18.0 nM 7.75
CHEMBL1095285 IC50 = 21.0 nM 7.68
CHEMBL235157 IC50 = 60.0 nM 7.22