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Assay Detail

CHEMBL1104503

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of LPS-induced MK2 phosphorylation in human whole blood treated 30 mins before LPS challenge measured after 45 mins by FACS analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

MAP kinase-activated protein kinase 2 (CHEMBL2208)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and evaluation of 7-alkyl-1,5-bis-aryl-pyrazolopyridinones as highly potent, selective, and orally efficacious inhibitors of p38alpha mitogen-activated protein kinase.
Pettus LH, Wurz RP, Xu S, Herberich B, Henkle B, Liu Q, McBride HJ, Mu S, Plant MH, Saris CJ, Sherman L, Wong LM, Chmait S, Lee MR, Mohr C, Hsieh F, Tasker AS.
J Med Chem (2010) 53 : 2973 -2985
PubMed 20218619 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 8.25 8.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1089865 1 8.37
CHEMBL1091199 1 8.24
CHEMBL1091928 1 8.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1089865 IC50 = 4.3 nM 8.37
CHEMBL1091199 IC50 = 5.7 nM 8.24
CHEMBL1091928 IC50 = 7.0 nM 8.15