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Assay Detail

CHEMBL1106147

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CLR expressed in human HEK293 cells coexpressing human RAMP1 assessed as Inhibition of CGRP-induced cAMP production in the presence of 50% human serum
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Calcitonin gene-related peptide type 1 receptor (CHEMBL3798)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of potent, highly constrained CGRP receptor antagonists.
Stump CA, Bell IM, Bednar RA, Fay JF, Gallicchio SN, Hershey JC, Jelley R, Kreatsoulas C, Moore EL, Mosser SD, Quigley AG, Roller SA, Salvatore CA, Sharik SS, Theberge CR, Zartman CB, Kane SA, Graham SL, Selnick HG, Vacca JP, Williams TM.
Bioorg Med Chem Lett (2010) 20 : 2572 -2576
PubMed 20299218 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.69 9.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL512773 1 9.21
CHEMBL1089433 1 8.89
CHEMBL1092208 1 8.82
CHEMBL1092882 1 8.60
CHEMBL1092837 1 8.39
CHEMBL453643 1 8.27
CHEMBL1092838 1 8.19
CHEMBL1090848 1 7.85
CHEMBL1090841 1 7.37
CHEMBL1089772 1 7.36
CHEMBL1090847 1 7.09
CHEMBL1090515 1 7.02
CHEMBL223156 1 6.28
CHEMBL1090846 1 6.07
CHEMBL1092883 1 5.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL512773 IC50 = 0.62 nM 9.21
CHEMBL1089433 IC50 = 1.3 nM 8.89
CHEMBL1092208 IC50 = 1.5 nM 8.82
CHEMBL1092882 IC50 = 2.5 nM 8.60
CHEMBL1092837 IC50 = 4.1 nM 8.39
CHEMBL453643 IC50 = 5.4 nM 8.27
CHEMBL1092838 IC50 = 6.5 nM 8.19
CHEMBL1090848 IC50 = 14.0 nM 7.85
CHEMBL1090841 IC50 = 43.0 nM 7.37
CHEMBL1089772 IC50 = 44.0 nM 7.36
CHEMBL1090847 IC50 = 81.0 nM 7.09
CHEMBL1090515 IC50 = 95.0 nM 7.02
CHEMBL223156 IC50 = 530.0 nM 6.28
CHEMBL1090846 IC50 = 850.0 nM 6.07
CHEMBL1092883 IC50 = 1200.0 nM 5.92