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Assay Detail

CHEMBL1106332

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Brucella suis recombinant (6X)His tagged-beta carbonic anhydrase expressed in Escherichia coli BL21(DE3) after 15 mins by stopped flow carbon dioxide hydrase assay method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Brucella suis
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Inhibition studies of a beta-carbonic anhydrase from Brucella suis with a series of water soluble glycosyl sulfanilamides.
Vullo D, Nishimori I, Scozzafava A, Köhler S, Winum JY, Supuran CT.
Bioorg Med Chem Lett (2010) 20 : 2178 -2182
PubMed 20211561 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 7.25 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1089967 1 8.05
CHEMBL1090523 1 8.04
CHEMBL1093178 1 7.72
CHEMBL1090524 1 7.55
CHEMBL20 ACETAZOLAMIDE 4.0 1 7.20
CHEMBL1089637 1 7.19
CHEMBL1092670 1 7.17
CHEMBL1090522 1 6.99
CHEMBL1089968 1 6.96
CHEMBL21 SULFANILAMIDE 4.0 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1089967 Ki = 8.9 nM 8.05
CHEMBL1090523 Ki = 9.2 nM 8.04
CHEMBL1093178 Ki = 19.0 nM 7.72
CHEMBL1090524 Ki = 28.0 nM 7.55
CHEMBL20 ACETAZOLAMIDE Ki = 63.0 nM 7.20
CHEMBL1089637 Ki = 65.0 nM 7.19
CHEMBL1092670 Ki = 67.0 nM 7.17
CHEMBL1090522 Ki = 103.0 nM 6.99
CHEMBL1089968 Ki = 110.0 nM 6.96
CHEMBL21 SULFANILAMIDE Ki = 2500.0 nM 5.60