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Assay Detail

CHEMBL1106905

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]Tyr-ovine-CRF from CRF1 receptor in rat frontal cortex homogenate by gamma counting
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Frontal cortex
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Corticotropin-releasing factor receptor 1 (CHEMBL4649)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and structure-activity relationships of N3-pyridylpyrazinones as corticotropin-releasing factor-1 (CRF1) receptor antagonists.
Hartz RA, Ahuja VT, Schmitz WD, Molski TF, Mattson GK, Lodge NJ, Bronson JJ, Macor JE.
Bioorg Med Chem Lett (2010) 20 : 1890 -1894
PubMed 20176478 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 8.19 9.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL574852 1 9.62
CHEMBL583387 1 9.52
CHEMBL1084423 1 9.17
CHEMBL1089036 1 9.10
CHEMBL1077301 1 8.92
CHEMBL1089037 1 8.92
CHEMBL1086180 1 8.92
CHEMBL1086179 1 8.92
CHEMBL573748 1 8.82
CHEMBL559764 1 8.80
CHEMBL558280 1 8.77
CHEMBL1086181 1 8.68
CHEMBL1077249 1 8.57
CHEMBL1091818 1 8.54
CHEMBL1089038 1 8.33
CHEMBL1085930 1 8.17
CHEMBL1084413 1 7.96
CHEMBL1082241 1 7.92
CHEMBL1084414 1 7.77
CHEMBL1090739 1 7.51
CHEMBL1085683 1 7.19
CHEMBL1090740 1 7.05
CHEMBL1090738 1 6.85
CHEMBL1077299 1 6.62
CHEMBL1085931 1 6.14
CHEMBL1084422 1 6.13

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL574852 IC50 = 0.24 nM 9.62
CHEMBL583387 IC50 = 0.3 nM 9.52
CHEMBL1084423 IC50 = 0.68 nM 9.17
CHEMBL1089036 IC50 = 0.8 nM 9.10
CHEMBL1077301 IC50 = 1.2 nM 8.92
CHEMBL1089037 IC50 = 1.2 nM 8.92
CHEMBL1086180 IC50 = 1.2 nM 8.92
CHEMBL1086179 IC50 = 1.2 nM 8.92
CHEMBL573748 IC50 = 1.5 nM 8.82
CHEMBL559764 IC50 = 1.6 nM 8.80
CHEMBL558280 IC50 = 1.7 nM 8.77
CHEMBL1086181 IC50 = 2.1 nM 8.68
CHEMBL1077249 IC50 = 2.7 nM 8.57
CHEMBL1091818 IC50 = 2.9 nM 8.54
CHEMBL1089038 IC50 = 4.7 nM 8.33
CHEMBL1085930 IC50 = 6.8 nM 8.17
CHEMBL1084413 IC50 = 11.0 nM 7.96
CHEMBL1082241 IC50 = 12.0 nM 7.92
CHEMBL1084414 IC50 = 17.0 nM 7.77
CHEMBL1090739 IC50 = 31.0 nM 7.51
CHEMBL1085683 IC50 = 65.0 nM 7.19
CHEMBL1090740 IC50 = 90.0 nM 7.05
CHEMBL1090738 IC50 = 140.0 nM 6.85
CHEMBL1077299 IC50 = 240.0 nM 6.62
CHEMBL1085931 IC50 = 720.0 nM 6.14
CHEMBL1084422 IC50 = 740.0 nM 6.13