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Assay Detail

CHEMBL1107423

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human recombinant kappa opioid receptor expressed in HEK293 cells assessed as stimulation of [35S]GTPgammaS binding after 45 mins by microplate luminescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kappa-type opioid receptor (CHEMBL237)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Conformationally constrained kappa receptor agonists: stereoselective synthesis and pharmacological evaluation of 6,8-diazabicyclo[3.2.2]nonane derivatives.
Geiger C, Zelenka C, Lehmkuhl K, Schepmann D, Englberger W, Wünsch B.
J Med Chem (2010) 53 : 4212 -4222
PubMed 20441176 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 6.50 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL440765 U-69593 1 7.10
CHEMBL1094880 1 7.06
CHEMBL1095521 1 6.09
CHEMBL1094237 1 5.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL440765 U-69593 EC50 = 80.0 nM 7.10
CHEMBL1094880 EC50 = 87.0 nM 7.06
CHEMBL1095521 EC50 = 820.0 nM 6.09
CHEMBL1094237 EC50 = 1800.0 nM 5.75